Miller B R, DeFoliart G R, Yuill T M
Am J Trop Med Hyg. 1979 Sep;28(5):897-901.
La Crosse (LAC) virus filial infection rates were 0% for 279 first ovarian cycle larvae, 43% for 380 second ovarian cycle larvae, and 58% for 363 third ovarian cycle larvae from orally infected mosquitoes representing 14 Wisconsin populations of Aedes triseriatus. LAC virus was not detected in 72 pools representing 2,250 first ovarian cycle larvae, while 35 pools and 16 pools each containing 30 second and third ovarian cycle larvae, respectively, were all positive for LAC virus. Similar results were obtained when the extrinsic incubation period temperature was 25 degrees C, 27 degrees C, or variable (17, 23, and 29 degrees C for 8 hours each). LAC virus was not detected in 240 second ovarian cycle larvae in which the bloodmeal for the first ovarian cycle was non-infectious. Infection was not detected in 337 first ovarian cycle larvae from female mosquitoes that had been injected intrathoracically with LAC virus concomitantly with receiving a non-infectious bloodmeal. After an extrinsic incubation temperature of 25 degrees C, LAC virus was discovered in dissected mosquito ovarian tissue 7 days postfeeding on an infectious bloodmeal. The epidemiological implications of these findings are discussed.
来自威斯康星州14个三带喙库蚊种群的经口感染蚊子的279只首次卵巢周期幼虫的拉克罗斯（LAC）病毒子代感染率为0%，380只第二次卵巢周期幼虫的感染率为43%，363只第三次卵巢周期幼虫的感染率为58%。在代表2250只首次卵巢周期幼虫的72个样本池中未检测到LAC病毒，而分别含有30只第二次和第三次卵巢周期幼虫的35个样本池和16个样本池均检测出LAC病毒呈阳性。当外在潜伏期温度为25摄氏度、27摄氏度或可变温度（17、23和29摄氏度各8小时）时，也获得了类似结果。在首次卵巢周期血餐无传染性的240只第二次卵巢周期幼虫中未检测到LAC病毒。在首次卵巢周期接受非传染性血餐并同时经胸内注射LAC病毒的雌蚊的337只首次卵巢周期幼虫中未检测到感染。在25摄氏度的外在潜伏期温度下，在喂食传染性血餐7天后，在解剖的蚊子卵巢组织中发现了LAC病毒。讨论了这些发现的流行病学意义。
