C58 脾细胞增殖反应的特征：年龄依赖性变化。
Characterization of the blastogenic response of C58 spleen cells: age-dependent changes.
作者信息
Lawton J W, Murphy W H
出版信息
Immunology. 1974 Jun;26(6):1093-9.
Abstract
Optimum conditions were determined for the stimulation of DNA synthesis in C58 spleen cells by phytohaemagglutinin and allogeneic BALB spleen cells. Maximum stimulation occurred in 3 days in both cases. The rate of DNA synthesis was constant over a 4-hour labelling period with tritiated thymidine. DNA synthesis was significantly enhanced by 15 mM bicarbonate. The peak response of C58 spleen cells to PHA and allogeneic BALB spleen cells occurred when the mice were 3–7 months old; it decreased thereafter. This finding is discussed in relation to age-dependent immune polioencephalomyelitis in C58 mice.
摘要
确定了植物血凝素和同种异体BALB脾细胞刺激C58脾细胞中DNA合成的最佳条件。在这两种情况下，最大刺激均在3天内出现。在4小时的氚标记胸腺嘧啶核苷标记期内，DNA合成速率保持恒定。15 mM碳酸氢盐显著增强了DNA合成。C58脾细胞对PHA和同种异体BALB脾细胞的峰值反应出现在小鼠3至7个月大时；此后反应下降。结合C58小鼠中与年龄相关的免疫性脑脊髓灰质炎对这一发现进行了讨论。
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