Beskina S R, Barkhatova O I, Popov V L, Shatkin A A, Prozorovskiĭ S V
Antibiotiki. 1979 Aug;24(8):598-603.
Monolayer cultures of L-cells (mouse fibroblasts) were inoculated with the causative agent of paratrachoma (strain LB-I). Simultaneously penicillin in doses of 0.01, 0.1, 1, 10, 25, 50, 100, 150 and 200 microgram/ml was added and its effect on the causative agent in the infection dynamics (18, 24, 48 and 72 hours after the inoculation) was studied with the light and electron microscopes. Gradual changes in the ultrastructure of the vegetative forms were observed by the 24th hour with the use of penicillin in a dose of 0.01 microgram/ml: the size of the vegetative forms increased, the cell wall membranes separated and periplasmic space significantly enlarged, the protoplast fragments disjoined into it, large forms vacuolized and were fragment with membranes, sometimes multilayer ones. When the culture was exposed to large doses of penicillin, the rate of the changes in the structure was higher and they were simultaneously of several types. Various types of the changes and possible modes of their formation were analyzed. Morphologically they are similar to the processes observed in L-transformation of bacteria. However, these structures were not infectious.
用副沙眼病原体（LB-I株）接种L细胞（小鼠成纤维细胞）的单层培养物。同时加入剂量为0.01、0.1、1、10、25、50、100、150和200微克/毫升的青霉素，并利用光学显微镜和电子显微镜研究其在感染动态过程中（接种后18、24、48和72小时）对病原体的影响。在接种后24小时，使用剂量为0.01微克/毫升的青霉素时，观察到营养型超微结构的逐渐变化：营养型的大小增加，细胞壁膜分离，周质空间显著扩大，原生质体碎片分离进入其中，大型形态空泡化并被膜分隔，有时是多层膜。当培养物暴露于大剂量青霉素时，结构变化的速度更快，且同时呈现多种类型。分析了各种类型的变化及其可能的形成方式。在形态学上，它们类似于在细菌L型转化中观察到的过程。然而，这些结构没有传染性。
