Barkhatova O I, Popov V L, Kekcheeva N K, Prozorovskiĭ S V
Antibiotiki. 1984 Aug;29(8):580-5.
The possibility of L-transformation for two species of Rickettsia of the tick group (R. conorii and R. akari) was studied with light and electron microscopy. The experiments were performed on the cultures of L-cells (murine fibroblasts). Penicillin and vancomycin were used as the transforming agents. Electron microscopy revealed clear changes in the morphological structure of both species when the doses of penicillin and vancomycin were 50-100 and 250-1500 micrograms/ml, respectively. The antibiotics had a morphologically analogous effect on both species of Rickettsia. The resulting morphological structures were large oval spheroplast-like cells with significant expansion of the periplasmic space up to 1 X 1.75 micron under the effect of penicillin and 0.5 X 0.7 micron under the effect of vancomycin. R. akari was somewhat more sensitive to the antibiotics. Electron microscopy of the morphological changes in the Rickettsia induced by penicillin and vancomycin showed that they were similar to the changes observed in gram-negative bacteria on their L-transformation and to the penicillin forms of chlamydia (galprovia) and R. prowazekii. They may be considered as a phase of unbalanced growth and represent the initial stage of L-transformation.
利用光学显微镜和电子显微镜研究了蜱群立克次氏体的两个种（康氏立克次氏体和阿氏立克次氏体）发生L型转化的可能性。实验在L细胞（小鼠成纤维细胞）培养物上进行。青霉素和万古霉素用作转化剂。当青霉素和万古霉素的剂量分别为50 - 100微克/毫升和250 - 1500微克/毫升时，电子显微镜观察到这两个种的形态结构均有明显变化。这两种抗生素对两种立克次氏体的形态学作用相似。所形成的形态结构是大的椭圆形原生质球样细胞，在青霉素作用下，周质空间显著扩大至1×1.75微米，在万古霉素作用下为0.5×0.7微米。阿氏立克次氏体对这些抗生素稍敏感一些。对青霉素和万古霉素诱导的立克次氏体形态变化进行电子显微镜观察表明，它们类似于革兰氏阴性菌L型转化时所观察到的变化，也类似于衣原体（沙原衣原体）和普氏立克次氏体的青霉素形态。它们可被视为生长不平衡阶段，代表L型转化的初始阶段。
