Schesser J H, Bulla L A
Appl Environ Microbiol. 1979 May;37(5):1012-5. doi: 10.1128/aem.37.5.1012-1015.1979.
Toxicity of Bacillus thuringiensis parasporal crystals to the Indian meal moth, Plodia interpunctella, is described. The numbers of insects killed were in relation to crystal dry weight. Mortality was determined by comparing adult emergence in diets treated with crystals to emergence in untreated diets. There was only a 30% survival at an application of 0.414 microgram/cm2, and the mean 50% lethal concentration value was found to be 0.299 microgram/cm2. The use of emergence data has provided a reliable and reproducible bioassay for comparing relative toxicities of crystals, spores, and other cellular components to this economically important insect.
描述了苏云金芽孢杆菌伴孢晶体对印度谷螟(Plodia interpunctella)的毒性。死亡昆虫数量与晶体干重有关。通过比较用晶体处理的饲料中的成虫羽化情况与未处理饲料中的成虫羽化情况来确定死亡率。在施用0.414微克/平方厘米时,存活率仅为30%,发现平均半数致死浓度值为0.299微克/平方厘米。利用羽化数据为比较晶体、孢子和其他细胞成分对这种具有经济重要性的昆虫的相对毒性提供了一种可靠且可重复的生物测定方法。