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Immobilization of urease by thiol-disulphide interchange with concomitant purification.

作者信息

Carlsson J, Axén R, Brocklehurst K, Crook E M

出版信息

Eur J Biochem. 1974 May 2;44(1):189-94. doi: 10.1111/j.1432-1033.1974.tb03472.x.

DOI:10.1111/j.1432-1033.1974.tb03472.x
PMID:4852353
Abstract
摘要

相似文献

1
Immobilization of urease by thiol-disulphide interchange with concomitant purification.通过硫醇-二硫化物交换固定脲酶并同时进行纯化。
Eur J Biochem. 1974 May 2;44(1):189-94. doi: 10.1111/j.1432-1033.1974.tb03472.x.
2
A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe.一种通过共价色谱法从刀豆中制备高活性脲酶的简便方法,以及用2,2'-二吡啶二硫化物作为硫醇滴定剂和反应性探针研究其硫醇基团。
Biochem J. 1976 Nov;159(2):245-57. doi: 10.1042/bj1590245.
3
Anomalous interaction of 2-thiopyridone with proteins during thiol-disulfide interchange reactions.
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4
Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.蛋白质巯基化与可逆蛋白质-蛋白质偶联。N-琥珀酰亚胺基3-(2-吡啶二硫基)丙酸酯,一种新型异双功能试剂。
Biochem J. 1978 Sep 1;173(3):723-37. doi: 10.1042/bj1730723.
5
Use of thiol-disulphide interchange reactions in preparative work on plasma proteins.硫醇 - 二硫化物交换反应在血浆蛋白制备工作中的应用。
J Chromatogr. 1978 Feb 21;159(1):25-31. doi: 10.1016/s0021-9673(00)98543-1.
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PREPARATION OF AN INSULIN A CHAIN DERIVATIVE WITH AN INTACT INTRA-CHAIN DISULPHIDE BOND.具有完整链内二硫键的胰岛素A链衍生物的制备
Nature. 1963 Sep 14;199:1095-6. doi: 10.1038/1991095a0.
7
Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.L-麦角硫因及其他一些氨基硫酮与2,2'-和4,4'-联吡啶二硫化物的反应以及L-麦角硫因与碘乙酰胺的反应。2-巯基咪唑、2-和4-硫代吡啶酮、硫脲和硫代乙酰胺作为高反应性中性硫亲核试剂。
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The affinity of flavin semiquinones for certain aromatic compounds and disulfides.
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9
[On the temperature dependency of enzymatic reactions, IV. Hydrolysis of urea by urease].[论酶促反应的温度依赖性,IV. 脲酶催化尿素水解]
Hoppe Seylers Z Physiol Chem. 1967 Nov;348(11):1372-6.
10
Reactivity of the thiol group in human and bovine albumin at pH 3--9, as measured by exchange with 2,2'-dithiodipyridine.通过与2,2'-二硫代二吡啶交换来测定人血清白蛋白和牛血清白蛋白中硫醇基团在pH 3至9条件下的反应活性。
Eur J Biochem. 1980 May;106(1):291-5. doi: 10.1111/j.1432-1033.1980.tb06022.x.

引用本文的文献

1
A rapid and specific method for isolation of thiol-containing peptides from large proteins by thiol-disulfide exchange on a solid support.一种通过在固体支持物上进行硫醇-二硫键交换从大蛋白质中分离含硫醇肽段的快速且特异的方法。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3029-33. doi: 10.1073/pnas.72.8.3029.
2
Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.蛋白质巯基化与可逆蛋白质-蛋白质偶联。N-琥珀酰亚胺基3-(2-吡啶二硫基)丙酸酯,一种新型异双功能试剂。
Biochem J. 1978 Sep 1;173(3):723-37. doi: 10.1042/bj1730723.
3
A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe.
一种通过共价色谱法从刀豆中制备高活性脲酶的简便方法,以及用2,2'-二吡啶二硫化物作为硫醇滴定剂和反应性探针研究其硫醇基团。
Biochem J. 1976 Nov;159(2):245-57. doi: 10.1042/bj1590245.
4
A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.一种对硫醇基团具有绝对和选择性特异性的报告基团递送系统以及一种含有7-硝基苯并-2-恶唑-1,3-二氮杂环戊二烯部分的改进型荧光探针。
Biochem J. 1975 Nov;151(2):417-32. doi: 10.1042/bj1510417.