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乙肝抗原制剂的主要极性脂质:存在糖鞘脂的证据。

Major polar lipids of hepatitis B antigen preparations: evidence for the presence of a glycosphingolipid.

作者信息

Steiner S, Huebner M T, Dreesman G R

出版信息

J Virol. 1974 Sep;14(3):572-7. doi: 10.1128/JVI.14.3.572-577.1974.

DOI:10.1128/JVI.14.3.572-577.1974
PMID:4853069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC355551/
Abstract

The major phospholipids of hepatitis B antigen, subtype adw, were characterized as phosphatidylcholine, sphingomyelin, and lysophosphatidylcholine. In addition, two carbohydrate-containing lipids were observed, one of which was characterized as a non-sialic acid-containing, water-soluble glycosphingolipid. Upon hydrolysis the glycosphingolipid yielded both hydroxy and non-hydroxy fatty acid as well as a long-chain base which was putatively identified as dihydrosphingosine.

摘要

乙肝抗原adw亚型的主要磷脂被鉴定为磷脂酰胆碱、鞘磷脂和溶血磷脂酰胆碱。此外,还观察到两种含碳水化合物的脂质,其中一种被鉴定为不含唾液酸的水溶性糖鞘脂。该糖鞘脂水解后产生羟基脂肪酸和非羟基脂肪酸以及一种长链碱,推测该长链碱为二氢鞘氨醇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e737/355551/def1a58e69f5/jvirol00249-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e737/355551/def1a58e69f5/jvirol00249-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e737/355551/def1a58e69f5/jvirol00249-0178-a.jpg

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本文引用的文献

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J Lipid Res. 1964 Jan;5:126-7.
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THIN-LAYER CHROMATOGRAPHIC SEPARATION OF SPHINGOSINE AND RELATED BASES.鞘氨醇及相关碱基的薄层色谱分离
J Lipid Res. 1963 Jan;4:106-8.
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Phosphorus assay in column chromatography.柱色谱法中的磷测定
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Multiple chemical forms of hepatitis B surface antigen produced in yeast.酵母中产生的多种化学形式的乙型肝炎表面抗原。
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Cell-mediated immunity in guinea pigs to subunits derived from hepatitis B surface antigen.豚鼠对乙型肝炎表面抗原衍生亚单位的细胞介导免疫。
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Comparative biophysical studies of hepatitis B antigen, subtypes adw and ayw.乙型肝炎抗原adw和ayw亚型的比较生物物理研究
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Proc Natl Acad Sci U S A. 1977 Apr;74(4):1530-4. doi: 10.1073/pnas.74.4.1530.
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