通过光氧化反应测定的木瓜蛋白酶和菠萝蛋白酶组氨酸残基电离的正常表观pKa值。 - Suppr | 超能文献

Normal apparent pKa value for the ionization of the histidine residue of papain and stem bromelain as determined by photooxidation reaction.

作者信息

Murachi T, Okumura K

出版信息

FEBS Lett. 1974 Mar 15;40(1):127-9. doi: 10.1016/0014-5793(74)80909-9.

DOI:10.1016/0014-5793(74)80909-9

Abstract

摘要

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1

Normal apparent pKa value for the ionization of the histidine residue of papain and stem bromelain as determined by photooxidation reaction.通过光氧化反应测定的木瓜蛋白酶和菠萝蛋白酶组氨酸残基电离的正常表观pKa值。

FEBS Lett. 1974 Mar 15;40(1):127-9. doi: 10.1016/0014-5793(74)80909-9.

2

Photooxidation of histidine and tryptophan residues of papain in the presence of methylene blue.在亚甲蓝存在的情况下木瓜蛋白酶组氨酸和色氨酸残基的光氧化作用

J Biochem. 1975 May;77(5):913-8. doi: 10.1093/oxfordjournals.jbchem.a130815.

3

Isolation and characterization of a protease inhibitor from commercial stem bromelain acetone powder.

J Supramol Struct. 1973;1(3):249-54. doi: 10.1002/jss.400010309.

4

Ficin and papain inhibitor from chicken egg white.来自鸡蛋白的无花果蛋白酶和木瓜蛋白酶抑制剂。

Arch Biochem Biophys. 1968 Apr;125(1):367-75. doi: 10.1016/0003-9861(68)90672-3.

5

Photosensitized inactivation of stem bromelain. Oxidation of histidine, methionine, and tryptophan residues.茎菠萝蛋白酶的光敏失活。组氨酸、甲硫氨酸和色氨酸残基的氧化。

Biochemistry. 1975 Jan 28;14(2):249-55. doi: 10.1021/bi00673a009.

6

The case for assigning a value of approximately 4 to pKa-i of the essential histidine-cysteine interactive systems of papain, bromelain and ficin.关于将木瓜蛋白酶、菠萝蛋白酶和无花果蛋白酶的必需组氨酸 - 半胱氨酸相互作用系统的pKa-i值设定为约4的理由。

FEBS Lett. 1975 Feb 15;50(3):365-8. doi: 10.1016/0014-5793(75)80529-1.

7

The amino acid sequence around the active-site cysteine and histidine residues of stem bromelain.茎菠萝蛋白酶活性位点半胱氨酸和组氨酸残基周围的氨基酸序列。

Biochem J. 1970 Apr;117(2):341-6. doi: 10.1042/bj1170341.

8

Characterization of the acidic and basic limbs of a bell-shaped pH profile in the inhibitory activity of bromelain inhibitor VI.菠萝蛋白酶抑制剂VI抑制活性中钟形pH曲线的酸性和碱性部分的表征

Biopolymers. 2006 Mar;81(4):309-19. doi: 10.1002/bip.20419.

9

Inhibition of stem bromelain by the chloromethyl ketone derivatives of N-tosyl-L-phenylalanine and N-tosyl-L-lysine.

J Biochem. 1967 Nov;62(5):627-9. doi: 10.1093/oxfordjournals.jbchem.a128716.

10

Evidence for histidine in the active sites of ficin and stem-bromelain.无花果蛋白酶和茎菠萝蛋白酶活性位点中组氨酸的证据。

Biochem J. 1968 Nov;110(1):53-7. doi: 10.1042/bj1100053.

引用本文的文献

1

4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain.4-氯-7-硝基苯并-2-恶唑-1,3-二氮唑作为研究硫醇蛋白酶的反应性探针。有证据表明，无花果蛋白酶和菠萝蛋白酶可能缺乏与木瓜蛋白酶天冬氨酸-158构象相当的羧基。

Biochem J. 1976 Nov;159(2):235-44. doi: 10.1042/bj1590235.

2

Substrate specificity and modifications of the active centre of elastase-like neutral proteinases from horse blood leucocytes.马血白细胞中类弹性蛋白酶中性蛋白酶活性中心的底物特异性及修饰

Biochem J. 1976 Feb 1;153(2):397-402. doi: 10.1042/bj1530397.