无花果蛋白酶和茎菠萝蛋白酶活性位点中组氨酸的证据。
Evidence for histidine in the active sites of ficin and stem-bromelain.
作者信息
Husain S S, Lowe G
出版信息
Biochem J. 1968 Nov;110(1):53-7. doi: 10.1042/bj1100053.
Abstract
- Ficin and stem-bromelain are irreversibly inhibited by 1,3-dibromoacetone, a reagent designed to react first with the active-site cysteine residue and subsequently with a second nucleophile. Evidence is presented that establishes that a histidine residue is within a 5A locus of the active-site cysteine residue in both enzymes. The histidine residue in both enzymes is alkylated at N-1 by dibromoacetone. It is suggested that, as with papain, the thiol and imidazole groups act in concert in the hydrolysis of substrates by these enzymes. 2. The inhibition of thiol-subtilisin with 1,3-dibromoacetone is shown to be due to the alkylation of a cysteine residue only.
摘要
- 无花果蛋白酶和茎菠萝蛋白酶会被1,3 - 二溴丙酮不可逆地抑制，1,3 - 二溴丙酮是一种设计用于首先与活性位点半胱氨酸残基反应，随后与第二个亲核试剂反应的试剂。有证据表明，在这两种酶中，一个组氨酸残基位于活性位点半胱氨酸残基的5埃范围内。这两种酶中的组氨酸残基在N - 1位被二溴丙酮烷基化。有人提出，与木瓜蛋白酶一样，巯基和咪唑基团在这些酶水解底物的过程中协同作用。2. 结果表明，1,3 - 二溴丙酮对巯基枯草杆菌蛋白酶的抑制作用仅归因于一个半胱氨酸残基的烷基化。
相似文献
1
Evidence for histidine in the active sites of ficin and stem-bromelain.无花果蛋白酶和茎菠萝蛋白酶活性位点中组氨酸的证据。
Biochem J. 1968 Nov;110(1):53-7. doi: 10.1042/bj1100053.
2
Evidence for histidine in the active site of papain.木瓜蛋白酶活性位点中组氨酸的证据。
Biochem J. 1968 Aug;108(5):855-9. doi: 10.1042/bj1080855.
3
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain.4-氯-7-硝基苯并-2-恶唑-1,3-二氮唑作为研究硫醇蛋白酶的反应性探针。有证据表明，无花果蛋白酶和菠萝蛋白酶可能缺乏与木瓜蛋白酶天冬氨酸-158构象相当的羧基。
Biochem J. 1976 Nov;159(2):235-44. doi: 10.1042/bj1590235.
4
The amino acid sequence around the active-site cysteine and histidine residues of stem bromelain.茎菠萝蛋白酶活性位点半胱氨酸和组氨酸残基周围的氨基酸序列。
Biochem J. 1970 Apr;117(2):341-6. doi: 10.1042/bj1170341.
5
The location of the active-site histidine residue in the primary sequence of papain.木瓜蛋白酶一级序列中活性位点组氨酸残基的位置。
Biochem J. 1968 Aug;108(5):861-6. doi: 10.1042/bj1080861.
6
The amino acid sequence around the active-site cysteine and histidine residues, and the buried cysteine residue in ficin.无花果蛋白酶活性位点半胱氨酸和组氨酸残基周围的氨基酸序列，以及埋藏的半胱氨酸残基。
Biochem J. 1970 Apr;117(2):333-40. doi: 10.1042/bj1170333.
7
Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin.木瓜蛋白酶中可能在无花果蛋白酶中没有类似情况的双态转变的证据。木瓜蛋白酶和无花果蛋白酶活性中心中阳离子位点和疏水结合区域分布的差异。
Biochem J. 1980 Dec 1;191(3):707-18. doi: 10.1042/bj1910707.
8
The nature of general base-general acid catalysis in serine proteases.
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1335-42. doi: 10.1073/pnas.64.4.1335.
9
Reaction of glyceraldehyde-3-phosphate dehydrogenase with dibromoacetone.3-磷酸甘油醛脱氢酶与二溴丙酮的反应
Biochemistry. 1972 Sep 26;11(20):3753-62. doi: 10.1021/bi00770a013.
10
Evaluation of benzofuroxan as a chromophoric oxidizing agent for thiol groups by using its reactions with papain, ficin, bromelain and low-molecular-weight thiols.通过苯并呋咱与木瓜蛋白酶、无花果蛋白酶、菠萝蛋白酶及低分子量硫醇的反应来评估其作为硫醇基团发色氧化剂的性能。
Biochem J. 1977 Mar 1;161(3):627-37. doi: 10.1042/bj1610627.
引用本文的文献
1
Avian 3-hydroxy-3-methylglutaryl-CoA lyase: sensitivity of enzyme activity to thiol/disulfide exchange and identification of proximal reactive cysteines.禽源3-羟基-3-甲基戊二酰辅酶A裂解酶：酶活性对硫醇/二硫键交换的敏感性及近端反应性半胱氨酸的鉴定
Protein Sci. 1992 Sep;1(9):1144-53. doi: 10.1002/pro.5560010908.
本文引用的文献
1
A study of the number and reactivity of the sulfhydryl groups of ficin.无花果蛋白酶巯基的数量及反应活性研究。
Biochim Biophys Acta. 1961 Oct 28;53:332-42. doi: 10.1016/0006-3002(61)90445-0.
2
PAPAIN-CATALYSED HYDROLYSIS OF SOME HIPPURIC ESTERS. A NEW MECHANISM FOR PAPAIN-CATALYSED HYDROLYSIS.木瓜蛋白酶催化某些马尿酸酯的水解。木瓜蛋白酶催化水解的新机制。
Biochem J. 1965 Jul;96(1):199-204. doi: 10.1042/bj0960199.
3
THE CHEMICAL REACTIVITY OF THE THIOL GROUP IN THE ACTIVE CENTRE OF FICIN.无花果蛋白酶活性中心硫醇基团的化学反应性
Biochim Biophys Acta. 1964 Oct 23;92:111-24. doi: 10.1016/0926-6569(64)90275-5.
4
CURRENT STATUS OF THE STRUCTURE OF PAPAIN: THE LINEAR SEQUENCE, ACTIVE SULFHYDRYL GROUP, AND THE DISULFIDE BRIDGES.木瓜蛋白酶结构的当前状态：线性序列、活性巯基和二硫键。
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1276-83. doi: 10.1073/pnas.52.5.1276.
5
AMINO ACID COMPOSITION OF STEM BROMELAIN.茎菠萝蛋白酶的氨基酸组成
Biochemistry. 1964 Jul;3:932-4. doi: 10.1021/bi00895a014.
6
FICUS ENZYMES. I. SEPARATION OF THE PROTEOLYTIC ENZYMES OF FICUS CARICA AND FICUS GLABRATA LATICES.榕属植物酶。一、无花果和光滑榕乳汁中蛋白水解酶的分离
J Biol Chem. 1964 Jul;239:2170-7.
7
ION-EXCHANGE CHROMATOGRAPHY OF AMINO-ACIDS: IMPROVEMENTS IN THE SINGLE COLUMN SYSTEM.氨基酸的离子交换色谱法：单柱系统的改进
Nature. 1964 Aug 1;203:483-4. doi: 10.1038/203483a0.
8
Alkylation and identification of the histidine residues at the active site of ribonuclease.核糖核酸酶活性位点组氨酸残基的烷基化及鉴定
J Biol Chem. 1963 Jul;238:2413-9.
9
The mechanism of ficin-catalysed reactions.无花果蛋白酶催化反应的机制。
Biochem J. 1959 Jun;72(2):349-57. doi: 10.1042/bj0720349.
10
The oxidation of ribonuclease with performic acid.用过甲酸氧化核糖核酸酶。
J Biol Chem. 1956 Apr;219(2):611-21.
Zotero 插件