大鼠脾脏硫酸乙酰肝素硫酸酯酶活性的研究。该酶的细胞内分布及特性。
Studies on the heparin sulphamidase activity from rat spleen. Intracellular distribution and characterization of the enzyme.
作者信息
Friedman Y, Arsenis C
出版信息
Biochem J. 1974 Jun;139(3):699-708. doi: 10.1042/bj1390699.
Abstract
A sulphamidase activity present in rat spleen capable of hydrolysing N-[(35)S]sulphated heparin was studied. This activity was associated with the lysosomal fraction. Studies in vivo showed that the rat is capable of significantly desulphating heparin. Lysosomes in all the major tissues can effectively accumulate heparin. The heparin sulphamidase and arylsulphatase activities from rat spleen were separated by isoelectric focusing. Heparin sulphamidase was a distinct entity from all the arylsulphatase activities.
摘要
对大鼠脾脏中一种能够水解N-[(35)S]硫酸化肝素的硫酸酰胺酶活性进行了研究。该活性与溶酶体部分相关。体内研究表明，大鼠能够显著地使肝素脱硫。所有主要组织中的溶酶体都能有效地积累肝素。通过等电聚焦分离了大鼠脾脏中的肝素硫酸酰胺酶和芳基硫酸酯酶活性。肝素硫酸酰胺酶与所有芳基硫酸酯酶活性是不同的实体。
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