大鼠肝脏实质细胞和其他细胞中芳基硫酸酯酶A和B的相对分布。
Relative distribution of arylsulphatases A and B in rat liver parenchymal and other cells.
作者信息
Hook G E, Dodgson K S, Rose F A, Worwood M
出版信息
Biochem J. 1973 May;134(1):191-5. doi: 10.1042/bj1340191.
Abstract
The relative activities of arylsulphatases A and B were measured in rat liver parenchymal and non-parenchymal cells, in peritoneal macrophages and in a number of rat tissues. Although absolute values cannot be obtained, it was shown that the arylsulphatase B/arylsulphatase A activity ratio is much higher in non-parenchymal cells than in parenchymal cells. The ratios in adrenals, brain and testis are very similar to each other but differ from those found in spleen, kidney and liver. These ratio variations may be caused by alterations in the activity of the B enzyme rather than the A enzyme. The relatively high B enzyme/A enzyme ratios in all rat tissues explains why the method devised for the independent assay of human arylsulphatases A and B cannot be employed with rat tissues.
摘要
在大鼠肝实质细胞和非实质细胞、腹膜巨噬细胞以及多种大鼠组织中测定了芳基硫酸酯酶A和B的相对活性。尽管无法获得绝对值，但结果表明，非实质细胞中芳基硫酸酯酶B/芳基硫酸酯酶A的活性比值远高于实质细胞。肾上腺、脑和睾丸中的该比值彼此非常相似，但与脾脏、肾脏和肝脏中的不同。这些比值变化可能是由B酶而非A酶的活性改变引起的。所有大鼠组织中相对较高的B酶/A酶比值解释了为何为独立检测人芳基硫酸酯酶A和B而设计的方法不能用于大鼠组织。
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