瘤胃厌氧菌琥珀酸弧菌产生L-天冬酰胺酶。
L-Asparaginase production by the rumen anaerobe Vibrio succinogenes.
作者信息
Kafkewitz D, Goodman D
出版信息
Appl Microbiol. 1974 Jan;27(1):206-9. doi: 10.1128/am.27.1.206-209.1974.
Abstract
The rumen anaerobe Vibrio succinogenes possesses a constitutive L-asparaginase. The amount of enzyme produced is affected by the compound supplied to the organism to generate the fumaric acid it requires as a terminal electron acceptor. When nitrate is provided as the terminal electron acceptor, the amount of enzyme produced is affected by the compound provided to satisfy the nutritional requirement of the organism for succinic acid. Specific activities of up to 8.4 IU/mg of protein in cell-free extracts have been obtained. This specific activity is higher than has been previously reported for any organism. The enzyme has an apparent K(m) of 1.7 x 10(-5) M and low activity towards L-glutamine when assayed at pH 8.5.
摘要
瘤胃厌氧菌产琥珀酸弧菌拥有一种组成型L-天冬酰胺酶。所产生的酶量受供给该生物体以生成其所需的富马酸作为末端电子受体的化合物的影响。当提供硝酸盐作为末端电子受体时，所产生的酶量受为满足该生物体对琥珀酸的营养需求而提供的化合物的影响。在无细胞提取物中已获得高达8.4 IU/mg蛋白质的比活性。该比活性高于先前报道的任何生物体。该酶在pH 8.5下测定时的表观K(m)为1.7×10(-5) M，对L-谷氨酰胺的活性较低。
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