体液免疫刺激。I. 用抗三硝基苯（TNP）抗体处理的TNP细胞对（125I）碘脱氧尿苷和（3H）胸腺嘧啶核苷摄取增加。

Humoral immunostimulation. I. Increased uptake of (125I)iododeoxyuridine and (3H)thymidine into TNP-cells treated with anti-TNP antibody.

作者信息

Shearer W T, Philpott G W, Parker C W

出版信息

J Exp Med. 1974 Feb 1;139(2):367-79. doi: 10.1084/jem.139.2.367.

DOI:10.1084/jem.139.2.367

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2139524/

Abstract

Interaction of microgram quantities of highly purified rabbit anti-TNP antibodies with TNP-substituted HeLa, HEp-2, and L cells caused an intense stimulation of radioactive nucleoside ([(125)I]UdR and [(3)H]TdR) uptake which was maximal 24-72 h after exposure of cells to antibody. The stimulation of nucleoside uptake and presumaly DNA synthesis was shown to be immuno logically mediated because unsubstituted cells were not stimulated by anti-TNP antibody, normal rabbit gamma globulin did not stimulate TNP-cells, and a hapten inhibitor, epsilon-DNP-lysine, prevented the stimulation of TNP-cells by anti-TNP antibody. These findings demonstrate that interaction of antibody with cell surface antigen can alter cell membrane transport, and possibly can enhance cell growth.

摘要

微克量的高度纯化兔抗三硝基苯（TNP）抗体与TNP取代的HeLa细胞、HEp - 2细胞和L细胞相互作用，可强烈刺激放射性核苷（[¹²⁵I]尿苷（UdR）和[³H]胸腺嘧啶核苷（TdR））摄取，细胞接触抗体后24 - 72小时摄取量达到最大值。核苷摄取的刺激以及推测的DNA合成被证明是由免疫介导的，因为未被取代的细胞不会被抗TNP抗体刺激，正常兔γ球蛋白不会刺激TNP细胞，并且一种半抗原抑制剂ε - DNP - 赖氨酸可阻止抗TNP抗体对TNP细胞的刺激。这些发现表明抗体与细胞表面抗原的相互作用可改变细胞膜转运，并且可能增强细胞生长。

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STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM.家兔淋巴细胞的体外研究。I. 用抗独特型血清刺激母细胞转化

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