Hamdy F, Sevoian M, Holt S C
Infect Immun. 1974 Apr;9(4):740-9. doi: 10.1128/iai.9.4.740-749.1974.
The kinetic events involved in Marek's disease herpesvirus infection of avian cell culture were investigated by assaying viral infectivity and antigenicity as well as by electron microscopy during the infectious cycle. The levels of viral infectivity and complement-fixing (CF) antigens revealed that the rates of appearance of infectious particles and CF antigens were not synchronous. Viral specific CF antigen could be detected 5 h after infection, whereas viral infectivity or the appearance of viral particles could be demonstrated only after 10 h of infection. High proportions of the recovered CF antigens during the various stages of the infectious cycle were found to be soluble and did not sediment with the virus particles. Cytological analysis of the developmental stages of the JM virus-infected cells by thin sectioning and electron microscopy revealed that at 8 h small particles approximately 35 nm in diameter appeared in the cell nuclei. The appearance of nucleocapsids occurred at 10 h, and these were of varying shapes; however, all were approximately 100 nm in diameter. At approximately 18 h postinfection, mature virus particles were observed. Viral maturation of the immature particles occurred by the acquisition of envelope from the inner leaflet of the nuclear membrane or from the cytoplasmic membrane of the cell.
通过在感染周期中检测病毒感染性和抗原性以及借助电子显微镜，研究了马立克氏病疱疹病毒感染禽细胞培养物所涉及的动力学事件。病毒感染性水平和补体结合（CF）抗原显示，感染性颗粒和CF抗原的出现速率不同步。感染后5小时可检测到病毒特异性CF抗原，而病毒感染性或病毒颗粒的出现仅在感染10小时后才能证明。在感染周期的各个阶段，发现回收的CF抗原中有很大比例是可溶的，并且不会与病毒颗粒一起沉淀。通过超薄切片和电子显微镜对JM病毒感染细胞的发育阶段进行细胞学分析表明，在8小时时，细胞核中出现了直径约35nm的小颗粒。核衣壳在10小时出现，其形状各异；然而，所有核衣壳直径均约为100nm。在感染后约18小时，观察到成熟病毒颗粒。未成熟颗粒通过从核膜内小叶或细胞的细胞质膜获取包膜而发生病毒成熟。
