• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

在采用三种血清处理程序的风疹血凝抑制试验中胰蛋白酶处理的人红细胞的相容性

Compatibility of trypsin-modified human erythrocytes in the rubella hemagglutination-inhibition test employing three serum treatment procedures.

作者信息

Nelson D B, Quirin P, Inhorn S L

出版信息

Appl Microbiol. 1974 Apr;27(4):767-70. doi: 10.1128/am.27.4.767-770.1974.

DOI:10.1128/am.27.4.767-770.1974
PMID:4856950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380132/
Abstract

Results of comparative tests using trypsin-modified human type O erythrocytes and cells from newly hatched chickens with three standard serum treatment methods for rubella hemagglutination-inhibition techniques are reported. The kaolin, heparin-manganous chloride and dextran sulfate-calcium chloride methods could all be used with both cell types. Reproducibility with heparin-manganous chloride and dextran sulfate-calcium chloride was excellent with both cell types. Both methods gave generally higher antibody titers than the kaolin procedure. However, the use of human cells resulted in a more sensitive system than chicken cells with all serum treatment methods.

摘要

报告了使用胰蛋白酶修饰的人O型红细胞和新孵化鸡的细胞,采用三种标准血清处理方法进行风疹血凝抑制技术的对比试验结果。高岭土法、肝素-氯化锰法和硫酸葡聚糖-氯化钙法均可用于这两种细胞类型。肝素-氯化锰法和硫酸葡聚糖-氯化钙法对这两种细胞类型的重复性都很好。这两种方法产生的抗体效价通常都高于高岭土法。然而,在所有血清处理方法中,使用人细胞比鸡细胞产生的系统更敏感。

相似文献

1
Compatibility of trypsin-modified human erythrocytes in the rubella hemagglutination-inhibition test employing three serum treatment procedures.在采用三种血清处理程序的风疹血凝抑制试验中胰蛋白酶处理的人红细胞的相容性
Appl Microbiol. 1974 Apr;27(4):767-70. doi: 10.1128/am.27.4.767-770.1974.
2
Rubella hemagglutination inhibition: removal of nonspecific agglutination due to manganous chloride.风疹血凝抑制:去除因氯化锰导致的非特异性凝集。
Appl Microbiol. 1974 Dec;28(6):992-4. doi: 10.1128/am.28.6.992-994.1974.
3
Modified hemagglutination-inhibition test for rubella employing human group O erythrocytes.采用人O型红细胞的风疹改良血凝抑制试验。
Appl Microbiol. 1972 Mar;23(3):471-5. doi: 10.1128/am.23.3.471-475.1972.
4
Measurement of rubella antibody by hemagglutination inhibition. II. Characteristics of an improved HAI test employing a new method for the removal of non-immunoglobulin HA inhibitors from serum.
J Immunol. 1970 Apr;104(4):826-34.
5
Contribution to the serologic diagnosis of rubella. Application of a modified hemagglutination and hemagglutination inhibition method.对风疹血清学诊断的贡献。改良血凝及血凝抑制方法的应用。
Rev Roum Inframicrobiol. 1971;8(3):163-7.
6
Improved dextran sulfate-calcium chloride method for the removal of nonspecific inhibitors with modifications for nonspecific agglutinin removal in the rubella hemagglutination inhibition test.改良的硫酸葡聚糖-氯化钙法用于去除非特异性抑制剂,并对风疹血凝抑制试验中去除非特异性凝集素的方法进行了改进。
Appl Microbiol. 1972 Aug;24(2):264-9. doi: 10.1128/am.24.2.264-269.1972.
7
[The hemagglutination inhibition reaction in the diagnosis of rubella. Apropos of the critical and comparative study of 252 serums treated by heparin-MnCl2 and allowed to react with trypsin treated human O erytrocytes].
Ann Biol Clin (Paris). 1974;32(5):433-8.
8
Use of trypsin-modified human erythrocytes in rubella hemagglutination-inhibition testing.胰蛋白酶修饰的人红细胞在风疹血凝抑制试验中的应用。
Appl Microbiol. 1972 Sep;24(3):353-7. doi: 10.1128/am.24.3.353-357.1972.
9
Use of sodium polyanetholesulfonate-CaCl2 for removal of serum nonspecific inhibitors of rubella hemagglutination: comparison with other polyanion-divalent cation combinations.使用聚茴香脑磺酸钠-氯化钙去除风疹血凝反应的血清非特异性抑制剂:与其他聚阴离子-二价阳离子组合的比较。
J Clin Microbiol. 1977 Oct;6(4):348-58. doi: 10.1128/jcm.6.4.348-358.1977.
10
Rubella haemagglutination inhibitors: their separation from antibodies.风疹血凝抑制物:它们与抗体的分离
Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):679-85. doi: 10.1111/j.1699-0463.1971.tb00096.x.

引用本文的文献

1
Rubella serology: a comparison of four methods for exclusion of non-specific serum inhibitors.风疹血清学:四种排除非特异性血清抑制剂方法的比较
J Hyg (Lond). 1981 Jun;86(3):315-27. doi: 10.1017/s0022172400069060.
2
Rubella virus hemagglutination with human and animal erythrocytes: effect of age and trypsinization.风疹病毒与人和动物红细胞的血凝反应:年龄及胰蛋白酶处理的影响
J Clin Microbiol. 1978 May;7(5):442-7. doi: 10.1128/jcm.7.5.442-447.1978.
3
Evaluation of a new test system for rubella haemagglutination inhibiting antibodies.风疹血凝抑制抗体新检测系统的评估
J Clin Pathol. 1977 Nov;30(11):1011-4. doi: 10.1136/jcp.30.11.1011.
4
Virus titrations using trypsin modified erythrocytes.使用胰蛋白酶修饰红细胞进行病毒滴定。
J Clin Pathol. 1977 Oct;30(10):993-5. doi: 10.1136/jcp.30.10.993.
5
Use of sodium polyanetholesulfonate-CaCl2 for removal of serum nonspecific inhibitors of rubella hemagglutination: comparison with other polyanion-divalent cation combinations.使用聚茴香脑磺酸钠-氯化钙去除风疹血凝反应的血清非特异性抑制剂:与其他聚阴离子-二价阳离子组合的比较。
J Clin Microbiol. 1977 Oct;6(4):348-58. doi: 10.1128/jcm.6.4.348-358.1977.

本文引用的文献

1
Growth of high titered rubella virus in roller bottle cultures of Vero cells.风疹病毒高滴度毒株在非洲绿猴肾细胞滚瓶培养物中的生长。
Proc Soc Exp Biol Med. 1969 Jan;130(1):12-4. doi: 10.3181/00379727-130-33477.
2
Modified hemagglutination-inhibition test for rubella employing human group O erythrocytes.采用人O型红细胞的风疹改良血凝抑制试验。
Appl Microbiol. 1972 Mar;23(3):471-5. doi: 10.1128/am.23.3.471-475.1972.
3
Improved dextran sulfate-calcium chloride method for the removal of nonspecific inhibitors with modifications for nonspecific agglutinin removal in the rubella hemagglutination inhibition test.改良的硫酸葡聚糖-氯化钙法用于去除非特异性抑制剂,并对风疹血凝抑制试验中去除非特异性凝集素的方法进行了改进。
Appl Microbiol. 1972 Aug;24(2):264-9. doi: 10.1128/am.24.2.264-269.1972.
4
Use of trypsin-modified human erythrocytes in rubella hemagglutination-inhibition testing.胰蛋白酶修饰的人红细胞在风疹血凝抑制试验中的应用。
Appl Microbiol. 1972 Sep;24(3):353-7. doi: 10.1128/am.24.3.353-357.1972.