Merkal R S, Curran B J
Appl Microbiol. 1974 Aug;28(2):276-9. doi: 10.1128/am.28.2.276-279.1974.
The cultural characteristics of newly isolated strains of Mycobacterium paratuberculosis on a variety of media were studied. The mycobactin dependence ascribed to this species of Mycobacterium was found to be easily circumvented by incorporation of 1% ferric ammonium citrate in serum or egg yolk media, but the earliest and most abundant growth occurred on serum medium that contained mycobactin and sodium pyruvate. In the presence of ferric ammonium citrate, respiration did not decrease during 5 days; but in the presence of all other agents studied, respiration decreased after the first day.
研究了新分离的副结核分枝杆菌菌株在多种培养基上的培养特性。发现通过在血清或蛋黄培养基中加入1%柠檬酸铁铵,很容易克服该分枝杆菌属所具有的对分枝杆菌生长素的依赖性,但最早且生长最旺盛的情况出现在含有分枝杆菌生长素和丙酮酸钠的血清培养基上。在有柠檬酸铁铵存在的情况下,5天内呼吸作用没有下降;但在所有其他所研究的试剂存在的情况下,第一天后呼吸作用就下降了。