Walwick E R, Brady J T, Kay R E
Appl Microbiol. 1967 Jul;15(4):885-92. doi: 10.1128/am.15.4.885-892.1967.
A procedure was developed to analyze the inactivation of coliphage T3 during freeze-drying and subsequent rehydration. The amount of gross disruption of the phage as compared with the amount of phage remaining intact was evaluated by cesium chloride density gradient centrifugation. The amount of phage material able to adsorb to host cells and the residual infectivity after the drying were also evaluated. These analyses made it possible to determine the amount of phage material (i) degraded to protein and nucleic acid, (ii) intact or largely intact, (iii) capable of adsorption on host cells, and (iv) infective. The capacities of casein hydrolysate, ascorbic acid, thiourea, bovine albumin, polyethyleneglycol, raffinose, inositol, and lipoproteins to protect T3 bacteriophage from the stress of freeze-drying were investigated.
开发了一种程序来分析冻干及随后复水过程中大肠杆菌噬菌体T3的失活情况。通过氯化铯密度梯度离心法评估噬菌体的总体破坏量与保持完整的噬菌体数量之比。还评估了干燥后能够吸附到宿主细胞上的噬菌体物质的量以及残余感染力。这些分析使得确定噬菌体物质的量成为可能：(i) 降解为蛋白质和核酸的；(ii) 完整或基本完整的；(iii) 能够吸附到宿主细胞上的；(iv) 具有感染性的。研究了酪蛋白水解物、抗坏血酸、硫脲、牛血清白蛋白、聚乙二醇、棉子糖、肌醇和脂蛋白保护T3噬菌体免受冻干应激的能力。
