γ射线照射对脱氧核糖核组蛋白引发核糖核酸聚合酶的影响。
The effects of gamma-irradiation on the priming by deoxyribonucleohistone of ribonucleic acid polymerase.
作者信息
Lloyd P H, Nicholson B H, Peacocke A R
出版信息
Biochem J. 1967 Sep;104(3):999-1003. doi: 10.1042/bj1040999.
Abstract
- The effect of gamma-irradiation of solutions of DNA and deoxyribonucleohistone (DNH) on their ability to prime the synthesis of RNA by DNA-dependent RNA polymerase has been studied. 2. The priming ability of both DNA and DNH decreased continuously with increasing radiation dose, but more rapidly with DNH. 3. These decreases have been compared with decreases in molecular weight and with the breakdown of the specific hydrogen-bonded structure of DNA. 4. It is concluded that a process was occurring during gamma-irradiation of DNH that, although involving a decrease in molecular weight, did not diminish and even enhanced its priming ability. This is consistent with previous physicochemical evidence that gamma-irradiation causes dissociation of histone from DNH.
摘要
- 研究了γ射线辐照DNA溶液和脱氧核糖核组蛋白（DNH）对其通过依赖DNA的RNA聚合酶引发RNA合成能力的影响。2. DNA和DNH的引发能力均随辐射剂量的增加而持续下降，但DNH下降得更快。3. 将这些下降情况与分子量的降低以及DNA特定氢键结构的破坏进行了比较。4. 得出的结论是，在DNH的γ射线辐照过程中发生了一个过程，该过程虽然涉及分子量的降低，但并未削弱甚至增强了其引发能力。这与先前的物理化学证据一致，即γ射线辐照会导致组蛋白从DNH中解离。
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引用本文的文献
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