支原体：实验室菌株的营养研究
Mycoplasma: nutritional studies of laboratory strains.
作者信息
Erno H, Plastridge W N, Tourtellotte M E
出版信息
Acta Vet Scand. 1967;8(2):111-22. doi: 10.1186/BF03547837.
Growth of 12 laboratory strains of Mycoplasma isolated from cattle, chicken, goat, man and sewage were studied on 20 different solid mediums and 9 liquid mediums. Growth on solid mediums was evaluated on the basis of appearance of visible colonies, while growth in liquid mediums was determined by light transmission technique. A solid medium of Bacto PPLO agar + Bacto PPLO serum fraction (1 %) + horse blood (5 %), and 0.005 % of 8 nucleosides (adenosine, cytidine, guanosine, deoxyadenosine, deoxycytidine, deoxyguanosine, thymidine, and uridine) was unsurpassed by any other medium. Studies of atmospheric requirements demonstrated that all strains grew in jars containing either air or nitrogen (100 %). One strain preferred an atmosphere with 10 % CO, while 3 strains were inhibited by this concentration of carbon dioxide.
对从牛、鸡、山羊、人及污水中分离出的12株支原体实验室菌株在20种不同固体培养基和9种液体培养基上的生长情况进行了研究。固体培养基上的生长情况根据可见菌落的出现来评估，而液体培养基中的生长情况则通过透光技术来测定。一种由细菌学PPLO琼脂+Bacto PPLO血清成分（1%）+马血（5%）以及0.005%的8种核苷（腺苷、胞苷、鸟苷、脱氧腺苷、脱氧胞苷、脱氧鸟苷、胸腺嘧啶核苷和尿苷）组成的固体培养基优于其他任何培养基。对气体需求的研究表明，所有菌株在装有空气或氮气（100%）的罐中均能生长。有1株菌株更喜欢含有10%二氧化碳的环境，而有3株菌株会受到这种二氧化碳浓度的抑制。
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