Erno H, Stipkovits L
Acta Vet Scand. 1973;14(3):436-49. doi: 10.1186/BF03547431.
A survey is given of presently known mycoplasmas of bovine source. Media and methods of cultivation are described. Cholesterol dependence, being the basis of division of Mycoplasmatales into Mycoplasmataceae and Acholeplasmataceae, was examined directly and also indirectly employing sensitivity tests to digitonin and sodium polyanethole sulphonate (SPS). All by now recognized species were found to be correctly classified in Mycoplasma and Acholeplasma, respectively. Of the unnamed “serogroups” 2 should be classified in the latter genus, while 6 serogroups were members of the genus Mycoplasma. Correlation was found between the digitonin test and the direct determination of cholesterol requirement, whereas this was not the case with the SPS test.
本文综述了目前已知的牛源支原体。描述了培养的培养基和方法。胆固醇依赖性是将支原体目分为支原体科和无胆甾原体科的基础,通过直接以及使用对洋地黄皂苷和聚茴香脑磺酸钠(SPS)的敏感性试验进行间接检测。现已发现,所有公认的物种分别在支原体属和无胆甾原体属中得到了正确分类。在未命名的“血清群”中,有2个应归入后一个属,而6个血清群是支原体属的成员。发现洋地黄皂苷试验与胆固醇需求的直接测定之间存在相关性,而SPS试验则不然。