通过凝胶过滤分离微粒体膜和游离多核糖体。
The separation of microsomal membranes and free polyribosomes by gel filtration.
作者信息
McCole N, Palmer D N, Williams D J
出版信息
Biochem J. 1979 May 15;180(2):437-9. doi: 10.1042/bj1800437.
Abstract
The integrity of polyribosome-membrane complexes in microsomal preparations is dependent on the centrifugal conditions used for their preparation. This paper describes techniques based on gel filtration that enable the rapid and gentle separation of microsomal membranes from both free polyribosomes and soluble protein.
摘要
微粒体制剂中多核糖体-膜复合物的完整性取决于制备它们时所用的离心条件。本文描述了基于凝胶过滤的技术，该技术能够快速、温和地从游离多核糖体和可溶性蛋白质中分离出微粒体膜。
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引用本文的文献
1
Preparation and properties of a complex from rat liver of polyribosomes with components of the cytoskeleton.大鼠肝脏多核糖体与细胞骨架成分复合物的制备及其性质
Biochem J. 1983 Oct 15;216(1):215-26. doi: 10.1042/bj2160215.
本文引用的文献
1
Isolation of rat liver microsomes by gel filtration.通过凝胶过滤法分离大鼠肝脏微粒体。
Anal Biochem. 1973 Aug;54(2):597-603. doi: 10.1016/0003-2697(73)90392-8.
2
Use of gel filtration to monitor ribosome-membrane interaction.使用凝胶过滤法监测核糖体与膜的相互作用。
Biochem Soc Trans. 1975;3(1):197-8. doi: 10.1042/bst0030197.
3
Purification of polyribosomes, 70S, monomers or ribosomal subunits by gel filtration.通过凝胶过滤法纯化多核糖体、70S核糖体、单体或核糖体亚基。
Acta Physiol Lat Am. 1976;26(5):330-6.
4
A sub-population of rat liver membrane-bound ribosomes that are detached in vitro by carcinogens and centrifugation.大鼠肝膜结合核糖体的一个亚群，其在体外可被致癌物和离心作用分离出来。
Biochem J. 1978 Oct 15;176(1):9-14. doi: 10.1042/bj1760009.
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