McCole N, Palmer D N, Williams D J
Biochem J. 1979 May 15;180(2):437-9. doi: 10.1042/bj1800437.
The integrity of polyribosome-membrane complexes in microsomal preparations is dependent on the centrifugal conditions used for their preparation. This paper describes techniques based on gel filtration that enable the rapid and gentle separation of microsomal membranes from both free polyribosomes and soluble protein.
微粒体制剂中多核糖体-膜复合物的完整性取决于制备它们时所用的离心条件。本文描述了基于凝胶过滤的技术，该技术能够快速、温和地从游离多核糖体和可溶性蛋白质中分离出微粒体膜。
