通过凝胶过滤法纯化多核糖体、70S核糖体、单体或核糖体亚基。
Purification of polyribosomes, 70S, monomers or ribosomal subunits by gel filtration.
作者信息
García-Patrone M, Perazzolo C A, Algranati I D
出版信息
Acta Physiol Lat Am. 1976;26(5):330-6.
PMID:802625
Abstract
Chromatography through Bio-Gel columns is a simple and rapid method for the purification of different ribosomal particles. This mild procedure, easily adapted to many purposes with good yields, can substitute the conventional sucrose gradient centrifugations, especially when the integrity of labile particles or complexes must be preserved.
摘要
通过生物凝胶柱进行色谱分离是一种简单快速的纯化不同核糖体颗粒的方法。这种温和的方法易于适用于多种目的，且产率良好，可替代传统的蔗糖梯度离心法，尤其是在必须保持不稳定颗粒或复合物完整性的情况下。
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