Isobe Y, Shimada Y
Cell Tissue Res. 1983;231(3):481-94. doi: 10.1007/BF00218107.
The present study describes an experimental approach whereby myofibrillogenesis and the three-dimensional arrangement of myofibrils present within cultured skeletal muscle cells can be examined using the scanning electron microscope. This procedure uses cells that have been cultured on gold-coated coverslips, and treated with Triton X-100 to extract the cell membrane and the soluble cytoplasm. Subsequent electroconductive staining by treatment with thiocarbohydrazide and osmium allows the myofibrils to be visualized. The images of myofibrils in various states of development observed by this method generally accords to those previously reported by transmission electron microscopy. Cell elongation and adhesion to the substrate causes mechanical stress from different directions which meet at branchings of the cultured myotubes. Many myofibrils are observed to run in the direction of the inferred stress lines.
本研究描述了一种实验方法，通过该方法可以使用扫描电子显微镜检查培养的骨骼肌细胞内肌原纤维的形成过程以及肌原纤维的三维排列。该程序使用在涂有金的盖玻片上培养的细胞，并用Triton X-100处理以提取细胞膜和可溶性细胞质。随后通过硫代碳酰肼和锇处理进行导电染色，使肌原纤维可视化。通过这种方法观察到的处于不同发育状态的肌原纤维图像通常与先前通过透射电子显微镜报告的图像一致。细胞伸长和对底物的粘附会产生来自不同方向的机械应力，这些应力在培养的肌管分支处交汇。观察到许多肌原纤维沿推断的应力线方向排列。
