氧化精胺血清结合及其对细胞增殖的强效G1期抑制作用的证据。
Evidence for serum binding of oxidized spermine and its potent G1-phase inhibition of cell proliferation.
作者信息
Gaugas J M, Dewey D L
出版信息
Br J Cancer. 1979 May;39(5):548-57. doi: 10.1038/bjc.1979.100.
Abstract
Serum polyamine oxidase (EC 1.4.3.4) is known to react in vitro with radio-labelled spermine4+ to produce di-oxidized spermine which must incorporate the label. Di-oxidized spermine was compatible with a radio-labelled compound2+ separated from the reaction mixture by ion-exchange chromatography. The compound was measured and had a half-life of about 2.3 h in tissue culture medium. It also rapidly and tightly bound to an unidentified serum component (gel-filtration chromatography indicated a complex of mol. wt 70,000) so that dissociation required treatment with strong acid (10N HCl). Findings suggest that the di-oxidized spermine, in either its free cationic or bound form, potently arrested cell proliferation. This arrest was non-cytotoxic and was confined to the G1 phase of the cell cycle. Products of di-oxidized spermine autodegradation, including trace amounts of stable and cytotoxic acrolein (arrested S phase), were unlikely to have contributed significantly to the arrest.
摘要
已知血清多胺氧化酶（EC 1.4.3.4）在体外与放射性标记的精胺4+反应，生成必须结合该标记的二氧代精胺。二氧代精胺与通过离子交换色谱从反应混合物中分离出的放射性标记化合物2+相符。对该化合物进行了测量，其在组织培养基中的半衰期约为2.3小时。它还能迅速且紧密地与一种未鉴定的血清成分结合（凝胶过滤色谱显示为分子量70,000的复合物），因此解离需要用强酸（10N HCl）处理。研究结果表明，二氧代精胺无论是以游离阳离子形式还是结合形式，都能有效地阻止细胞增殖。这种阻止作用无细胞毒性，且局限于细胞周期的G1期。二氧代精胺自降解的产物，包括痕量的稳定且具有细胞毒性的丙烯醛（阻止S期），不太可能对这种阻止作用有显著贡献。
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