el-Nahal H M
Bull World Health Organ. 1967;36(3):423-9.
In view of the morphological similarity between the blood stages of Plasmodium berghei berghei and P. b. yoelii on the one hand and P. vinckei and P. chabaudi on the other, these rodent malaria parasites were investigated by the indirect fluorescent antibody technique in the hope of thus differentiating between them.Sera were obtained from rats hyperimmunized against these four parasites, from rabbits immunized against the soluble antigens of the blood stages of the parasites, and from a rabbit immunized against the sporozoites of P. b. yoelii. The titre of each serum was determined by its reaction with the homologous and the heterologous antigens in a modified immunofluorescent technique.The results showed a definite cross-reaction between the four parasites, indicating a common antigen; no serological difference between P. b. berghei and P. b. yoelii (these being considered the berghei group or Group 1); no serological difference between P. vinckei and P. chabaudi (vinckei group or Group 2); and a definite serological difference between Group 1 and Group 2 infections.The theoretical antigenic structure of rodent plasmodia is discussed in the light of these findings.
鉴于一方面伯氏疟原虫（柏氏疟原虫）和约氏疟原虫的血液阶段在形态上相似，另一方面文氏疟原虫和查巴迪疟原虫在形态上相似，因此采用间接荧光抗体技术对这些啮齿动物疟原虫进行了研究，以期对它们进行区分。从对这四种寄生虫进行过超免疫的大鼠、对这些寄生虫血液阶段的可溶性抗原进行过免疫的兔子以及对约氏疟原虫子孢子进行过免疫的兔子身上获取血清。通过改良的免疫荧光技术，根据每种血清与同源抗原和异源抗原的反应来测定其滴度。结果显示这四种寄生虫之间存在明确的交叉反应，表明存在共同抗原；伯氏疟原虫和约氏疟原虫之间无血清学差异（这两种被视为伯氏疟原虫组或第1组）；文氏疟原虫和查巴迪疟原虫之间无血清学差异（文氏疟原虫组或第2组）；第1组和第2组感染之间存在明确的血清学差异。根据这些发现，对啮齿动物疟原虫的理论抗原结构进行了讨论。
