Ramsey J M, Beaudoin R L, Bawden M P, Espinal C A
Trans R Soc Trop Med Hyg. 1983;77(3):378-81. doi: 10.1016/0035-9203(83)90168-2.
The specific identification of plasmodial sporozoites is not possible on morphological grounds. This study presents a serological method for the identification of sporozoite species, indicating the suitability of this approach for detection and determination of sporozoites in wild vectors collected from malaria endemic areas. Specific antisera and monoclonal antibodies prepared against each of two species of rodent malaria (Plasmodium berghei or P. yoelii) were evaluated for their ability to discriminate among sporozoites of different malaria species (P. vivax, P. gallinaceum, P. berghei, P. yoelii) from varied host types. Antisera produced by intravenous immunization of rabbits or mice and hybridoma-produced monoclonal antibodies reacted only with homologous sporozoites in an immunofluorescent antibody test. Antisera produced by intravenous challenge showed no significant difference in reactivity with fresh sporozoites as compared with sporozoites frozen at -80 degrees C for up to six months, whereas antisera produced by infective mosquito bites showed reduced sensitivity to frozen-stored homologous sporozoites and some cross-reactions with heterologous sporozoites. Antisera prepared against the erythrocytic stages of P. berghei or P. yoelii also cross-reacted with sporozoites of all four plasmodia tested, indicating that it is advantageous to use anti-sporozoite antibodies for the identification of malaria sporozoite species by means of serological tests.
基于形态学依据无法对疟原虫的子孢子进行特异性鉴定。本研究提出了一种用于鉴定子孢子种类的血清学方法，表明该方法适用于检测和确定从疟疾流行地区采集的野生媒介中的子孢子。针对两种啮齿类疟疾（伯氏疟原虫或约氏疟原虫）制备的特异性抗血清和单克隆抗体，被评估用于区分来自不同宿主类型的不同疟原虫种类（间日疟原虫、鸡疟原虫、伯氏疟原虫、约氏疟原虫）子孢子的能力。通过静脉注射免疫兔或小鼠产生的抗血清以及杂交瘤产生的单克隆抗体，在免疫荧光抗体试验中仅与同源子孢子发生反应。静脉注射激发产生的抗血清与新鲜子孢子的反应性，与在-80℃冷冻长达六个月的子孢子相比，没有显著差异，而通过感染性蚊虫叮咬产生的抗血清对冷冻保存的同源子孢子敏感性降低，并且与异源子孢子存在一些交叉反应。针对伯氏疟原虫或约氏疟原虫红细胞阶段制备的抗血清，也与所测试的所有四种疟原虫的子孢子发生交叉反应，这表明通过血清学试验使用抗子孢子抗体来鉴定疟原虫子孢子种类是有利的。
