Kubilus J, MacDonald M J, Baden H P
Biochim Biophys Acta. 1979 Jun 19;578(2):484-92. doi: 10.1016/0005-2795(79)90178-8.
Bovine and human epidermal cells were cultured on mitomycin C treated fibroblasts. The cells were carried through four passages and found to synthesize fibrous proteins and insoluble cell envelopes. Acid buffer soluble fibrous protein, prekeratin, and urea soluble fibrous protein were both identified and the latter was the major component in older cultures. Some of the prekeratin polypeptides of intact tissue were not found in cultured cells, but the ones that were present corresponded to those of whole tissue. X-ray diffraction, amino acid analysis and immunological techniques were used to establish that the polypeptides were keratins. The insoluble cell envelopes had a higher proline and 1/2 cystine content than the fibrous protein, similar to what is found in whole epidermis. Histidase, a characteristic enzyme marker of whole epidermis, was not observed in cultured cells. These studies indicate that differentiation occurs in cultured cells but it may not be as complete as in intact tissue.
将牛和人的表皮细胞培养在经丝裂霉素C处理的成纤维细胞上。这些细胞传代培养了四代，发现它们能合成纤维状蛋白质和不溶性细胞包膜。鉴定出了酸缓冲液可溶性纤维状蛋白质、前角蛋白以及尿素可溶性纤维状蛋白质，后者是较老培养物中的主要成分。完整组织中的一些前角蛋白多肽在培养细胞中未被发现，但存在的那些与全组织中的相对应。使用X射线衍射、氨基酸分析和免疫技术确定这些多肽为角蛋白。不溶性细胞包膜的脯氨酸和半胱氨酸含量高于纤维状蛋白质，这与全表皮中的情况相似。在培养细胞中未观察到全表皮特有的酶标记物组氨酸酶。这些研究表明培养细胞中发生了分化，但可能不如完整组织中那样完全。
