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用荧光抗体技术诊断伪狂犬病

The diagnosis of pseudorabies by the fluorescent antibody technique.

作者信息

Meyling A, Bitsch V

出版信息

Acta Vet Scand. 1967;8(4):360-8. doi: 10.1186/BF03547822.

Abstract

Fluorescent antibody staining (FA) for pseudorabies has been performed on frozen sections from the cerebrum, cerebellum, and pons from 51 piglets suspected of pseudorabies. Pseudorabies virus could be demonstrated in the brains of 23 of these pigs both by FA and by rabbit and/or tissue culture inoculation. Most fluorescent cells were found in the cerebral cortex. It is concluded that FA staining may become a reliable alternative to already existing methods for diagnosis of pseudorabies.

摘要

对51头疑似感染伪狂犬病的仔猪的大脑、小脑和脑桥的冰冻切片进行了伪狂犬病荧光抗体染色(FA)。通过FA以及兔和/或组织培养接种,在其中23头猪的大脑中检测到了伪狂犬病病毒。大多数荧光细胞见于大脑皮层。结论是,FA染色可能成为现有伪狂犬病诊断方法的一种可靠替代方法。

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