Schmidt H, Bode R, Birnbaum D
Sektion Biologie, Ernst-Moritz-Arndt-Universität Greifswald, GDR.
Antonie Van Leeuwenhoek. 1989 Nov;56(4):337-47. doi: 10.1007/BF00443747.
The regulatory properties of four enzymes (homocitrate synthase, alpha-aminoadipate reductase, saccharopine reductase, saccharopine dehydrogenase) involved in the lysine biosynthesis of Pichia guilliermondii were investigated and compared with the regulatory patterns found in other yeast species. The first enzyme of the pathway, homocitrate synthase, is feedback-inhibited by L-lysine. Some other amino acids (alpha-aminoadipate, glutamate, tryptophan, leucine) and lysine analogues are also inhibitors of one or more enzymes. It is shown that only the synthesis of homocitrate synthase is weakly repressed by L-lysine.
研究了季也蒙毕赤酵母赖氨酸生物合成过程中涉及的四种酶（高柠檬酸合酶、α-氨基己二酸还原酶、酵母氨酸还原酶、酵母氨酸脱氢酶）的调控特性，并与其他酵母物种的调控模式进行了比较。该途径的第一种酶，即高柠檬酸合酶，受到L-赖氨酸的反馈抑制。其他一些氨基酸（α-氨基己二酸、谷氨酸、色氨酸、亮氨酸）和赖氨酸类似物也是一种或多种酶的抑制剂。结果表明，只有高柠檬酸合酶的合成受到L-赖氨酸的微弱抑制。
