LYC1是酵母中赖氨酸N-6-乙酰转移酶的结构基因。

LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast.

作者信息

Beckerich J M, Lambert M, Gaillardin C

机构信息

Laboratoire de Biologie Moléculaire et Cellulaire INRA-CNRS, Institut National Agronomique, Thiverval Grignon, France.

出版信息

Curr Genet. 1994 Jan;25(1):24-9. doi: 10.1007/BF00712962.

DOI:10.1007/BF00712962

PMID:8082161

Abstract

In the yeast Yarrowia lipolytica, the LYC1 locus controls the first step of the lysine degradation pathway which is catalyzed by lysine N-6-acetyl transferase (LAT). This gene was cloned by complementation of the lyc1-100 mutation. Its position in the cloned insert was determined by conversion mapping and by complementation. The LYC1 gene encodes a 391 amino-acid polypeptide which has no homolog in protein databases. The required upstream region extends over 960 bp. When placed under the control of the GAL10 promoter in Saccharomyces cerevisiae, LYC1 drives the expression of lysine acetyl transferase activity, thus providing strong evidence that it is the structural gene encoding this enzyme.

摘要

在解脂耶氏酵母中，LYC1基因座控制赖氨酸降解途径的第一步，该步骤由赖氨酸N-6-乙酰基转移酶（LAT）催化。通过lyc1-100突变的互补作用克隆了该基因。通过转化作图和互补作用确定了其在克隆插入片段中的位置。LYC1基因编码一个391个氨基酸的多肽，该多肽在蛋白质数据库中没有同源物。所需的上游区域延伸超过960 bp。当置于酿酒酵母中GAL10启动子的控制下时，LYC1驱动赖氨酸乙酰转移酶活性的表达，从而提供了强有力的证据表明它是编码该酶的结构基因。