调节基因产物（阻遏物）在大肠杆菌β-半乳糖苷酶合成的分解代谢阻遏中的作用。
Role of the regulator-gene product (repressor) in catabolite repression of beta-galactosidase synthesis in Escherichia coli.
作者信息
Palmer J, Moses V
出版信息
Biochem J. 1968 Jan;106(2):339-43. doi: 10.1042/bj1060339.
Abstract
- The specific role of the lac repressor (i-gene product) in transient catabolite repression evoked by the introduction of glucose into the medium has been investigated in Escherichia coli by using mutants of the i-gene. 2. A temperature-sensitive mutant (i(TL)) is normally inducible and demonstrates transient repression when grown at 32 degrees . At 42 degrees it is about 20% constitutive and transient catabolite repression is abolished. 3. A strain carrying an amber suppressor-sensitive mutation in the i-gene is phenotypically constitutive and also fails to show transient catabolite repression. 4. Insertion of Flaci(+) into this strain restores both inducibility and transient repression. 5. It is concluded that the i-gene product interacts with the catabolite co-repressor in such a way that its affinity for the operator is increased.
摘要
- 利用i基因的突变体，在大肠杆菌中研究了乳糖阻遏物（i基因产物）在向培养基中引入葡萄糖所引发的短暂分解代谢物阻遏中的具体作用。2. 一个温度敏感突变体（i(TL)）通常是可诱导的，在32摄氏度生长时表现出短暂阻遏。在42摄氏度时，它约20%组成型表达，且短暂分解代谢物阻遏被消除。3. 一个在i基因中携带琥珀抑制敏感突变的菌株在表型上是组成型的，并且也未表现出短暂分解代谢物阻遏。4. 将Flaci(+)插入该菌株可恢复诱导性和短暂阻遏。5. 得出的结论是，i基因产物与分解代谢物共阻遏物相互作用，其结果是增加了它对操纵基因的亲和力。
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