通过葡萄糖饥饿预处理筛选大肠杆菌温度敏感突变体。
Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation.
作者信息
Boyd D, Nixon R, Gillespie S, Gillespie D
出版信息
J Bacteriol. 1968 Mar;95(3):1040-50. doi: 10.1128/jb.95.3.1040-1050.1968.
Abstract
A system for screening Escherichia coli temperature-sensitive mutants is described. The system involves glucose starvation and minimizes ambiguities introduced by the interdependencies of macromolecular synthesis during balanced growth. The system permits the quick recognition of protein synthesis mutants and their classification into two general catagories. Complete protein synthesis mutants are unable to make any polypeptide material, whereas partial protein synthesis mutants are able to produce inactive proteins. The phenotypes of several mutants are described.
摘要
描述了一种用于筛选大肠杆菌温度敏感突变体的系统。该系统涉及葡萄糖饥饿，并最大限度地减少了平衡生长期间大分子合成相互依赖性所引入的模糊性。该系统允许快速识别蛋白质合成突变体并将其分为两个一般类别。完全蛋白质合成突变体无法产生任何多肽物质，而部分蛋白质合成突变体能够产生无活性的蛋白质。描述了几种突变体的表型。
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