Patterson D, Gillespie D
J Bacteriol. 1972 Dec;112(3):1177-83. doi: 10.1128/jb.112.3.1177-1183.1972.
Upon a temperature shift from 30 C to 42 to 44 C, Escherichia coli experiences a transient slowdown in rate of optical density increase, uridine incorporation, and amino acid incorporation. The data show that the primary target for thermal inactivation is a molecule(s) required for the initiation of protein synthesis. The effect on ribonucleic acid synthesis is a secondary effect.
当温度从30℃转变为42至44℃时,大肠杆菌在光密度增加速率、尿苷掺入以及氨基酸掺入方面经历短暂减缓。数据表明,热失活的主要靶点是蛋白质合成起始所需的一种或多种分子。对核糖核酸合成的影响是次要影响。