Massell B F, Michael J G, Amezcua J, Siner M
Appl Microbiol. 1968 Mar;16(3):509-18. doi: 10.1128/am.16.3.509-518.1968.
A partially purified M protein, extracted from a mouse-virulent strain of type 3, group A streptococcus, was administered subcutaneously in gradually increasing amounts at weekly intervals to 21 children in a Family Program. Seven children with type 3 bactericidal antibody in prevaccination sera showed a secondary response. Of 14 children with no detectable type 3 bactericidal antibody prior to vaccination, 13 developed definite type 3 antibody during or soon after vaccination. This response appeared to be of the primary variety in at least some of the 13 children because (i) the total incidence of antibody response (20 of 21) was greater than can be accounted for by the documented incidence of clinical and subclinical type 3 infections among children of our Family Program during a period of 14 years, (ii) the response in the 13 children with no detectable antibody prior to vaccination was more delayed than in those showing a definite secondary response, and (iii) on the average, the amount of vaccine needed for a response in these 13 children was 15 to 28 times greater than that required for the secondary response. Local skin reactions were variable among the vaccinated children. Systemic reactions were infrequent and not severe. The giving of multiple injections of partially purified M protein did not seem to cause cutaneous hypersensitivity.
从A组3型小鼠强毒株中提取的部分纯化M蛋白，以每周递增的剂量皮下注射给一个家庭计划中的21名儿童。7名接种前血清中有3型杀菌抗体的儿童出现了二次反应。在接种前未检测到3型杀菌抗体的14名儿童中，有13名在接种期间或接种后不久产生了明确的3型抗体。这种反应在至少部分13名儿童中似乎是初次反应类型，原因如下：（i）抗体反应的总发生率（21名中的20名）高于我们家庭计划中儿童在14年期间记录的临床和亚临床3型感染发生率所能解释的范围；（ii）接种前未检测到抗体的13名儿童的反应比出现明确二次反应的儿童更为延迟；（iii）平均而言，这13名儿童产生反应所需的疫苗量比二次反应所需的量高15至28倍。接种疫苗的儿童中局部皮肤反应各不相同。全身反应很少且不严重。多次注射部分纯化的M蛋白似乎不会引起皮肤超敏反应。
