Pittman Q J, Blume H W, Kearney R E, Renaud L P
Brain Res. 1979 Sep 28;174(1):39-53. doi: 10.1016/0006-8993(79)90802-3.
Extracellular action potentials were recorded from 1098 neurons in the medial hypothalamus of pentobarbital anesthetized male rats. Their excitability was analyzed after single 1 Hz stimulation of the midbrain periaqueductal gray (PAG) or adjacent reticular formation. Cells were also examined for their response to median eminence (ME), amygdala, lateral septum (LS) or anterior hypothalamic/preoptic area (AHA/POA) stimulation. Antidromic invasion from midbrain stimulation was recorded from 110 neurons. Eight of these neurons showed features of axon branching and displayed antidromic invasion from both midbrain and amygdala (2 cells) or AHA/POA (6 cells). Many neurons with midbrain projections displayed orthodromic responses to stimulation in the amygdala, but few responded to AHA/POA or LS stimulation. Midbrain stimulation evoked orthodromic responses from 99 medial hypothalamic neurons. Many of these cells also displayed orthodromic responses to amygdala or AHA/POA stimulation, whereas a small number were activated antidromically by stimulation in these sites. None of 42 neurons activated antidromically from median eminence stimulation were responsive to midbrain stimulation. These results provide electrophysiological evidence of reciprocal connections between medial hypothalamic and medial midbrain areas, and indicate that medial hypothalamic neurons with midbrain connections are subject to influences from other extrahypothalamic areas.
在戊巴比妥麻醉的雄性大鼠的内侧下丘脑，记录了1098个神经元的细胞外动作电位。在对中脑导水管周围灰质（PAG）或相邻网状结构进行单次1Hz刺激后，分析了它们的兴奋性。还检测了这些细胞对正中隆起（ME）、杏仁核、外侧隔（LS）或下丘脑前部/视前区（AHA/POA）刺激的反应。从110个神经元记录到了来自中脑刺激的逆向冲动。其中8个神经元表现出轴突分支的特征，并显示出来自中脑和杏仁核（2个细胞）或AHA/POA（6个细胞）的逆向冲动。许多有中脑投射的神经元对杏仁核刺激表现出顺向反应，但很少对AHA/POA或LS刺激有反应。中脑刺激诱发了99个内侧下丘脑神经元的顺向反应。这些细胞中的许多也对杏仁核或AHA/POA刺激表现出顺向反应，而少数细胞在这些部位的刺激下被逆向激活。从正中隆起刺激逆向激活的42个神经元中，没有一个对中脑刺激有反应。这些结果提供了内侧下丘脑和中脑内侧区域之间相互连接的电生理证据，并表明与中脑有连接的内侧下丘脑神经元受到其他下丘脑外区域的影响。
