Cotter D A, Raper K B
J Bacteriol. 1968 Jul;96(1):86-92. doi: 10.1128/jb.96.1.86-92.1968.
Washed spores of Dictyostelium discoideum, strains NC-4H, NC-4D, and V-12, germinated rapidly after being heat shocked at or near 45.0 C for 30 min. Cultures of the slime molds were grown in association with Escherichia coli B/r as the host bacterium; spores taken from plates of synthetic medium had a higher final germination value than spores from complex medium containing peptone and yeast extract. Young spores germinated more rapidly than older spores. Optimal germination occurred between pH 6.0 and 7.0, and, of the buffers tested, potassium phosphate allowed the most rapid germination. After heat shocking, spores were diluted into fresh oxygenated buffer to provide enough oxygen for completion of germination. Germination occurred most rapidly between incubation temperatures of 22 and 25 C.
盘基网柄菌（Dictyostelium discoideum）的洗涤孢子，菌株NC - 4H、NC - 4D和V - 12，在45.0℃或接近该温度下热激30分钟后迅速萌发。黏菌培养物与大肠杆菌B/r作为宿主细菌共同培养；从合成培养基平板上获取的孢子比含有蛋白胨和酵母提取物的复合培养基上的孢子具有更高的最终萌发值。年轻孢子比年老孢子萌发更快。在pH 6.0至7.0之间发生最佳萌发，并且在所测试的缓冲液中，磷酸钾能使孢子萌发最快。热激后，将孢子稀释到新鲜的含氧缓冲液中，以提供足够的氧气来完成萌发。在22至25℃的培养温度之间萌发最为迅速。
