Ennis H L, Sussman M
J Bacteriol. 1975 Oct;124(1):62-4. doi: 10.1128/jb.124.1.62-64.1975.
After activation, wild-type Dictyostelium discoideum spores germinate rapidly and synchronously in phosphate buffer as well as in complex medium. Mutants defective in spore germination were isolated and characterized. These mutants (called grm) did not germinate normally in buffer but did germinate in complex medium in the presence of bacteria. One mutant (grm B) swelled normally, but amoebae were not formed. Another mutant (grm F) swelled and germinated poorly in buffer. The members of the third group of mutants (A, C, D, and E) did not swell or give rise to amoebae in buffer.
激活后，野生型盘基网柄菌孢子在磷酸盐缓冲液以及复合培养基中能迅速且同步地萌发。分离并鉴定了孢子萌发存在缺陷的突变体。这些突变体（称为grm）在缓冲液中不能正常萌发，但在含有细菌的复合培养基中能够萌发。一个突变体（grm B）能正常膨胀，但不能形成变形虫。另一个突变体（grm F）在缓冲液中膨胀且萌发较差。第三组突变体（A、C、D和E）在缓冲液中既不膨胀也不产生变形虫。
