来自大肠杆菌的甲硫氨酰转移核糖核酸合成酶的亚基结构与特异性
Sub-unit structure and specificity of methionyl-transfer-ribonucleic acid synthetase from Escherichia coli.
作者信息
Bruton C J, Hartley B S
出版信息
Biochem J. 1968 Jun;108(2):281-8. doi: 10.1042/bj1080281.
Abstract
- The purification of methionyl-transfer-RNA synthetase from Escherichia coli by a modified technique gives a 16% yield of a protein that appears homogeneous by the criteria of disc gel electrophoresis, ultracentrifugation and end-group analysis. 2. The molecular weight is 96000 and the protein consists of two sub-units of 48000, which appear to be identical. The amino acid composition and thiol content are reported. 3. Kinetic data are reported for analogues of methionine and for pure t-RNA(F) and t-RNA(M), which are respectively the methionine transfer RNA that can exist in the formylmethionyl form and the one that can exist only in the methionyl form. The enzyme binds and acylates both species of transfer RNA identically.
摘要
- 用一种改良技术从大肠杆菌中纯化甲硫氨酰 - 转移RNA合成酶，所得蛋白质的产率为16%。根据圆盘凝胶电泳、超速离心和末端基团分析的标准，该蛋白质看起来是均一的。2. 分子量为96000，该蛋白质由两个48000的亚基组成，这两个亚基似乎是相同的。报告了其氨基酸组成和巯基含量。3. 报告了甲硫氨酸类似物以及纯的t - RNA(F)和t - RNA(M)的动力学数据，t - RNA(F)和t - RNA(M)分别是可以以甲酰甲硫氨酰形式存在的甲硫氨酸转移RNA和只能以甲硫氨酰形式存在的甲硫氨酸转移RNA。该酶对这两种转移RNA的结合和酰化作用相同。
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