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Methionyl-t-RNA synthetase (where t-RNA denotes ;soluble' or transfer RNA) has been purified to apparent homogeneity from a ribonuclease I-free strain of Escherichia coli. Polyacrylamide-gel electrophoresis of the final product revealed a single band. The purified enzyme catalyses the exchange of 450mumoles of pyrophosphate into ATP/mg. in 15min. at 37 degrees . 2. Methionyl-t-RNA synthetase is specific for the l-isomer of methionine, but appears to catalyse the methionylation of two distinct species of t-RNA, both of which are specific for methionine, but only one of which may be subsequently formylated. 3. The Michaelis constant for l-methionine is 2x10(-4)m in the ATP-PP(i) exchange assay and 2x10(-5)m for the acylation of t-RNA. 4. Gel filtration of both crude and highly purified preparations of methionyl-t-RNA synthetase on Sephadex G-200 indicates that the active species of enzyme has a molecular weight of about 190000. The amino acid composition of the enzyme is similar to those reported for the isoleucine and tyrosine enzymes from E. coli.

甲硫氨酰 - tRNA合成酶（其中tRNA表示“可溶性”或转运RNA）已从无核糖核酸酶I的大肠杆菌菌株中纯化至表观均一。最终产物的聚丙烯酰胺凝胶电泳显示为单一一条带。纯化后的酶在37摄氏度下15分钟内可催化450微摩尔焦磷酸与ATP/毫克发生交换。
甲硫氨酰 - tRNA合成酶对甲硫氨酸的L - 异构体具有特异性，但似乎催化两种不同种类的tRNA进行甲硫氨酰化，这两种tRNA都对甲硫氨酸具有特异性，但其中只有一种随后可能被甲酰化。
在ATP - PP(i)交换测定中，L - 甲硫氨酸的米氏常数为2×10(-4)摩尔，对于tRNA的酰化反应，米氏常数为2×10(-5)摩尔。
对粗制和高度纯化的甲硫氨酰 - tRNA合成酶制剂在葡聚糖G - 200上进行凝胶过滤表明，该酶的活性形式分子量约为190000。该酶的氨基酸组成与报道的来自大肠杆菌的异亮氨酸和酪氨酸合成酶的氨基酸组成相似。

Methionyl-t-RNA synthetase (where t-RNA denotes ;soluble' or transfer RNA) has been purified to apparent homogeneity from a ribonuclease I-free strain of Escherichia coli. Polyacrylamide-gel electrophoresis of the final product revealed a single band. The purified enzyme catalyses the exchange of 450mumoles of pyrophosphate into ATP/mg. in 15min. at 37 degrees . 2. Methionyl-t-RNA synthetase is specific for the l-isomer of methionine, but appears to catalyse the methionylation of two distinct species of t-RNA, both of which are specific for methionine, but only one of which may be subsequently formylated. 3. The Michaelis constant for l-methionine is 2x10(-4)m in the ATP-PP(i) exchange assay and 2x10(-5)m for the acylation of t-RNA. 4. Gel filtration of both crude and highly purified preparations of methionyl-t-RNA synthetase on Sephadex G-200 indicates that the active species of enzyme has a molecular weight of about 190000. The amino acid composition of the enzyme is similar to those reported for the isoleucine and tyrosine enzymes from E. coli.

甲硫氨酰 - tRNA合成酶（其中tRNA表示“可溶性”或转运RNA）已从无核糖核酸酶I的大肠杆菌菌株中纯化至表观均一。最终产物的聚丙烯酰胺凝胶电泳显示为单一一条带。纯化后的酶在37摄氏度下15分钟内可催化450微摩尔焦磷酸与ATP/毫克发生交换。
甲硫氨酰 - tRNA合成酶对甲硫氨酸的L - 异构体具有特异性，但似乎催化两种不同种类的tRNA进行甲硫氨酰化，这两种tRNA都对甲硫氨酸具有特异性，但其中只有一种随后可能被甲酰化。
在ATP - PP(i)交换测定中，L - 甲硫氨酸的米氏常数为2×10(-4)摩尔，对于tRNA的酰化反应，米氏常数为2×10(-5)摩尔。
对粗制和高度纯化的甲硫氨酰 - tRNA合成酶制剂在葡聚糖G - 200上进行凝胶过滤表明，该酶的活性形式分子量约为190000。该酶的氨基酸组成与报道的来自大肠杆菌的异亮氨酸和酪氨酸合成酶的氨基酸组成相似。

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