Kawaguchi T, Tsuchida T, Matsushita H
Clin Chim Acta. 1979 Mar 1;92(2):125-34. doi: 10.1016/0009-8981(79)90105-0.
Human sera from normal subjects and diabetic patients were electrophoresed in an urea-containing gel; polyacrylamide gel and cellulose acetate membrane. In polyacrylamide gel, normal serum exhibited the fast migrating Band 2 and the slower migrating albumin Bands 4 and 5 after overnight fasting and also after glucose administration. In diabetic serum Bands 4 and 5 did not appear before or after glucose administration in low mercaptoethanol gel which abnormality resembled the pattern in C57BL/KsJ-db/db mice. In high mercaptoethanol gel, about half of the diabetic sera exhibit a delay in appearance of Bands 4 and 5, i.e., Bands 4 and 5 were not observed 30--60 min after glucose administration, which seems to resemble the pattern in C57BL/6J-ob/ob mice. Conditions of electrophoresis in urea-submerged cellulose acetate membrane (species of buffer systems, pH, ion concentration, mercaptoethanol, ethylene-diaminetetraacetic acid, Ca2+ ion, urea concentration, etc.) were observed in relation to albumin sub-separation. At pH 8.6 with barbital buffer, albumin separated into two bands, and at pH 10.6 with glycine buffer, albumin separated into four bands. Almost all diabetic sera (ca. 80%) exhibited different electrophoretic patterns from that of normal serum.
对来自正常受试者和糖尿病患者的人血清在含尿素的凝胶（聚丙烯酰胺凝胶和醋酸纤维素膜）中进行电泳。在聚丙烯酰胺凝胶中，正常血清在禁食过夜后以及给予葡萄糖后均显示出快速迁移的条带2以及迁移较慢的白蛋白条带4和条带5。在糖尿病血清中，在低巯基乙醇凝胶中，无论在给予葡萄糖之前还是之后，条带4和条带5均未出现，这种异常类似于C57BL/KsJ-db/db小鼠中的模式。在高巯基乙醇凝胶中，约一半的糖尿病血清显示条带4和条带5出现延迟，即给予葡萄糖后30 - 60分钟未观察到条带4和条带5，这似乎类似于C57BL/6J-ob/ob小鼠中的模式。观察了醋酸纤维素膜浸没在尿素中时的电泳条件（缓冲系统种类、pH值、离子浓度、巯基乙醇、乙二胺四乙酸、Ca2+离子、尿素浓度等）与白蛋白亚分离的关系。在巴比妥缓冲液pH 8.6时，白蛋白分离为两条带，在甘氨酸缓冲液pH 10.6时，白蛋白分离为四条带。几乎所有糖尿病血清（约80%）显示出与正常血清不同的电泳模式。
