Cantafora A, Angelico M, Attili A F, Ercoli L, Capocaccia L
Clin Chim Acta. 1979 Aug 1;95(3):501-8. doi: 10.1016/0009-8981(79)90202-x.
This study has been aimed at improving some steps in the gas-liquid chromatographic determination of sulfated bile acids. The best conditions for the enzymatic hydrolysis with cholylglycine hydrolase of sulfolithocholylglycine and sulfolithocholyltaurine are described. Recoveries of more than 85% were obtained after prolonging the incubation to 12 h. A single-step procedure for solvolysis and methylation of bile acids was achieved by using, as a water scavenger, 2,2-dimethoxypropane added directly to the hydrolysis mixture. This procedure avoided the difficulty of the extraction of sulfated bile acids from aqueous solutions.
本研究旨在改进硫酸化胆汁酸气液色谱测定中的一些步骤。描述了用胆酰甘氨酸水解酶对磺基石胆酰甘氨酸和磺基石胆酰牛磺酸进行酶促水解的最佳条件。将孵育时间延长至12小时后，回收率超过85%。通过将2,2-二甲氧基丙烷直接添加到水解混合物中作为脱水剂，实现了胆汁酸溶剂解和甲基化的一步法。该方法避免了从水溶液中提取硫酸化胆汁酸的困难。
