Lepage G, Roy C C, Weber A M
J Lipid Res. 1981 May;22(4):705-11.
Methods currently in use for the quantitative measurement of sulfated serum bile acids usually lead to low recoveries. The present technique uses two internal standards and involves enzymatic hydrolysis followed by extraction of the non-sulfated fraction in ether at pH 4.6. The sulfated fraction is then solvolysed before gas-liquid chromatographic analysis of the individual bile acids of both fractions. Using 200 microliters of serum, recoveries of more than 81% of sulfated bile acids were achieved. This method is of particular significance in view of recent evidence showing that certain sulfated bile acids are cholestatic.
目前用于定量测定血清硫酸化胆汁酸的方法通常回收率较低。本技术使用两种内标，包括酶促水解，然后在pH 4.6条件下用乙醚萃取非硫酸化部分。硫酸化部分随后进行溶剂解，然后对两部分的各个胆汁酸进行气液色谱分析。使用200微升血清，硫酸化胆汁酸的回收率超过81%。鉴于最近有证据表明某些硫酸化胆汁酸具有胆汁淤积作用，该方法具有特别重要的意义。
