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Preparation and analysis of peptide fragments produced by pepsin hydrolysis of human plasma albumin and their relationship to its structure.胃蛋白酶水解人血浆白蛋白产生的肽片段的制备与分析及其与白蛋白结构的关系。
Biochem J. 1968 Aug;109(1):107-20. doi: 10.1042/bj1090107.
2
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6
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本文引用的文献

1
The spectrophotometric determination of tyrosine and tryptophan in proteins.蛋白质中酪氨酸和色氨酸的分光光度测定法。
Biochem J. 1946;40(5-6):628-32. doi: 10.1042/bj0400628.
2
A steam distillation apparatus suitable for micro-Kjeldahl analysis.一种适用于微量凯氏定氮分析的水蒸气蒸馏装置。
Biochem J. 1942 Dec;36(10-12):790-1. doi: 10.1042/bj0360790.
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Isolation and characterization of fragments of human serum albumin containing some of the antigenic sites of the whole molecule.含有全分子部分抗原位点的人血清白蛋白片段的分离与鉴定。
Biochem J. 1962 Apr;83(1):172-80. doi: 10.1042/bj0830172.
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Abnormal rotatory dispersion of proteins in the far ultraviolet.蛋白质在远紫外区的异常旋光色散
Arch Biochem Biophys. 1962 Feb;96:314-20. doi: 10.1016/0003-9861(62)90413-7.
5
Recent developments in techniques for terminal and sequence studies in peptides and proteins.肽和蛋白质末端及序列研究技术的最新进展。
Methods Biochem Anal. 1955;2:359-425. doi: 10.1002/9780470110188.ch12.
6
SUBTILISIN-CATALYZED HYDROLYSIS OF BOVINE PLASMA ALBUMIN: IMPLICATIONS WITH RESPECT TO A POSTULATED SUBUNIT MODEL.枯草杆菌蛋白酶催化的牛血浆白蛋白水解：关于一个假定的亚基模型的意义
Biochemistry. 1965 Apr;4:634-44. doi: 10.1021/bi00880a004.
7
PARTIAL CHARACTERIZATION OF HUMAN PEPSIN I, PEPSIN IIA, PEPSIN IIB, AND PEPSIN 3.人胃蛋白酶I、胃蛋白酶IIA、胃蛋白酶IIB和胃蛋白酶3的部分特性分析
Biochemistry. 1964 Sep;3:1203-9. doi: 10.1021/bi00897a003.
8
INCREASED GLOBULIN FRACTIONATION BY AN IMPROVED VERTICAL GEL ELECTROPHORETIC TECHNIQUE.通过改进的垂直凝胶电泳技术提高球蛋白分级分离效果。
J Lab Clin Med. 1964 Jul;64:133-44.
9
FRAGMENTATION OF BOVINE SERUM ALBUMIN BY PEPSIN. II. ISOLATION, AMINO ACID COMPOSITION, AND PHYSICAL PROPERTIES OF THE FRAGMENTS.胃蛋白酶对牛血清白蛋白的裂解。II. 裂解片段的分离、氨基酸组成及物理性质
J Biol Chem. 1964 May;239:1424-31.
10
FRAGMENTATION OF BOVINE SERUM ALBUMIN BY PEPSIN. I. THE ORIGIN OF THE ACID EXPANSION OF THE ALBUMIN MOLECULE.胃蛋白酶对牛血清白蛋白的裂解。I. 白蛋白分子酸性膨胀的起源。
J Biol Chem. 1964 May;239:1415-23.

胃蛋白酶水解人血浆白蛋白产生的肽片段的制备与分析及其与白蛋白结构的关系。

Preparation and analysis of peptide fragments produced by pepsin hydrolysis of human plasma albumin and their relationship to its structure.

作者信息

Franglen G, Swaniker G R

出版信息

Biochem J. 1968 Aug;109(1):107-20. doi: 10.1042/bj1090107.

DOI:10.1042/bj1090107
PMID:4876098
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186759/
Abstract

Human plasma albumin was prepared and subjected to proteolysis by pepsin at pH2.45 at 25 degrees for 10min. with albumin/pepsin ratio 3000:1. Five peptide fragments were detected in the proteolysate by means of zone electrophoresis and gel filtration; these were separated and purified. Molecular weights, amino acid composition and disulphide bond content of the purified fragments were determined. The results show that a high proportion of the polypeptide chain of albumin appears to have a low cystine content, and at low pH values the molecule would be expected to have a considerable degree of freedom in its structure in these regions of the chain. A tripartite model for the structure of plasma albumin is proposed.

摘要

制备人血浆白蛋白，并在25℃、pH2.45条件下用胃蛋白酶以白蛋白/胃蛋白酶比例3000:1进行蛋白水解10分钟。通过区带电泳和凝胶过滤在蛋白水解产物中检测到五个肽片段；将这些片段分离并纯化。测定了纯化片段的分子量、氨基酸组成和二硫键含量。结果表明，白蛋白多肽链的很大一部分似乎胱氨酸含量较低，在低pH值下，预计分子在链的这些区域其结构具有相当程度的自由度。提出了血浆白蛋白结构的三方模型。