Osebold J W, DiCapua R A
J Bacteriol. 1968 Jun;95(6):2158-64. doi: 10.1128/jb.95.6.2158-2164.1968.
The importance of bringing live bacteria into intimate contact with macrophages as a prerequisite for establishing cellular immunity was investigated. The bacterium Listeria monocytogenes was shown to replicate and survive in diffusion chambers implanted in the peritoneal cavities of mice. Humoral substances accruing from host responses to diffusing soluble antigens of the microorganism were unable to inactivate the bacteria. The resistance of mice immunized by subcutaneous inoculation of the live organism always exceeded the resistance of mice with Listeria diffusion chamber implants. Animals with sham diffusion chambers were more resistant to a challenge by L. monocytogenes than were normal mice. Host resistance was not significantly different between Listeria diffusion chamber implant groups and sham diffusion chamber implant groups. The results suggested that direct involvement of macrophages with the parasite is necessary to achieve cellular immunity.
研究了让活细菌与巨噬细胞密切接触作为建立细胞免疫前提条件的重要性。单核细胞增生李斯特菌被证明可在植入小鼠腹腔的扩散小室中复制并存活。宿主对微生物可溶性抗原扩散产生的体液物质无法使细菌失活。通过皮下接种活生物体免疫的小鼠的抵抗力始终超过植入李斯特菌扩散小室的小鼠的抵抗力。植入假扩散小室的动物比正常小鼠对单核细胞增生李斯特菌的攻击更具抵抗力。李斯特菌扩散小室植入组和假扩散小室植入组之间的宿主抵抗力没有显著差异。结果表明，巨噬细胞直接参与寄生虫感染对于实现细胞免疫是必要的。
