Hage-Chahine C M, Del Giudice G, Lambert P H, Pechere J C
Department of Genetics and Microbiology, University of Geneva Medical School, Switzerland.
Infect Immun. 1992 Apr;60(4):1415-21. doi: 10.1128/iai.60.4.1415-1421.1992.
A murine experimental infection with a hemolysin-producing (Hly+) strain of Listeria monocytogenes and a non-hemolysin-producing (Hly-) mutant was used as an in vivo model to evaluate the role of hemolysin production in the immune response. No antilisterial antibodies were detectable following sublethal infection with Hly+ bacteria, but consistent antilisterial immunoglobulin G (IgG) and IgM antibody production was observed following sublethal infection with the Hly- mutant. Hly+ but not Hly- L. monocytogenes induced transient inhibition of antibody response to Hly- bacteria and to unrelated T-cell-dependent (tetanus toxoid) and T-cell-independent (pneumococcal polysaccharide 3) antigens. Transient inhibition of the activation of an antigen-specific T-cell clone was also observed following Hly+ infection of antigen-presenting cells but not following Hly- infection. These results suggest that hemolysin production by L. monocytogenes is an important factor in modulating the immune response to T-cell-dependent and T-cell-independent antigens in infected individuals.
用产溶血素（Hly+）的单核细胞增生李斯特菌菌株和不产溶血素（Hly-）的突变体对小鼠进行实验性感染，以此作为体内模型来评估溶血素产生在免疫反应中的作用。用Hly+细菌进行亚致死感染后未检测到抗李斯特菌抗体，但用Hly-突变体进行亚致死感染后观察到持续的抗李斯特菌免疫球蛋白G（IgG）和IgM抗体产生。Hly+而非Hly-单核细胞增生李斯特菌诱导了对Hly-细菌以及对无关的T细胞依赖性（破伤风类毒素）和T细胞非依赖性（肺炎球菌多糖3）抗原的抗体反应的短暂抑制。在抗原呈递细胞被Hly+感染后也观察到了抗原特异性T细胞克隆激活的短暂抑制，而在被Hly-感染后未观察到。这些结果表明，单核细胞增生李斯特菌产生的溶血素是调节感染个体对T细胞依赖性和T细胞非依赖性抗原的免疫反应的一个重要因素。
