电离辐射对大肠杆菌B-r同步培养物的影响。
Effects of ionizing radiation on synchronous cultures of Escherichia coli B-r.
作者信息
Clark D J
出版信息
J Bacteriol. 1968 Oct;96(4):1150-8. doi: 10.1128/jb.96.4.1150-1158.1968.
Abstract
The sensitivity of Escherichia coli B/r to X-irradiation is correlated with the replication cycle of deoxyribonucleic acid (DNA). The sensitivity to X-irradiation in the wild type can be attributed to the presence of nuclear targets plus DNA repair mechanisms. The effects of nuclear targets are observed in the recombination-deficient (rec-) mutant B/r, but the sensitivity reflected by changes in the slope of killing curves is absent. A study of different growth conditions indicates that maximal resistance to X rays occurs toward the middle of the division cycle. Evidence is offered that branched chromosomes respond as one-hit targets to X-irradiation. The killing effects of heavy-ion bombardment on E. coli are due primarily to ionizing radiation.
摘要
大肠杆菌B/r对X射线的敏感性与脱氧核糖核酸（DNA）的复制周期相关。野生型对X射线的敏感性可归因于核靶点的存在以及DNA修复机制。在重组缺陷型（rec-）突变体B/r中可观察到核靶点的作用，但不存在杀伤曲线斜率变化所反映的敏感性。对不同生长条件的研究表明，对X射线的最大抗性出现在分裂周期的中期。有证据表明，分支染色体对X射线的反应如同单靶点。重离子轰击对大肠杆菌的杀伤作用主要归因于电离辐射。
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