Tasseron-De Jong J G, Brouwer J, Rietveld K, Zoetemelk C E, Bosch L
Eur J Biochem. 1979 Oct;100(1):271-83. doi: 10.1111/j.1432-1033.1979.tb02058.x.
Late after infection of KB cells with adenovirus 5 an extra protein becomes associated with messenger ribonucleoprotein particles present in the polysomes. This protein has a molecular weight of 100000 and is identical to the virus coded '100K' protein found previously. The extra protein is firmly attached to the messenger ribonucleoprotein complexes. Its binding resists exposure to high salt concentrations as used in puromycin/high-salt dissociation and equilibrium centrifugation in Cs2SO4 gradients. In this respect it resembles the binding of two other proteins of Mr 74000 and 48000 which are commonly found in messenger ribonucleoprotein particles of various eukaryotic cells. The identity between the messenger ribonucleoprotein protein of Mr 100000 and the "100K' protein present in the soluble part of the cytoplasm was established by sodium dodecylsulphate/polyacrylamide gel electrophoresis, isoelectric focusing and peptide mapping after limited proteolysis with Staphylococcus aureus protease.
用腺病毒5感染KB细胞后晚期，一种额外的蛋白质与多核糖体中存在的信使核糖核蛋白颗粒结合。这种蛋白质的分子量为100000，与先前发现的病毒编码的“100K”蛋白质相同。这种额外的蛋白质牢固地附着在信使核糖核蛋白复合物上。其结合能抵抗在嘌呤霉素/高盐解离和Cs2SO4梯度平衡离心中使用的高盐浓度。在这方面，它类似于另外两种分子量分别为74000和48000的蛋白质的结合，这两种蛋白质常见于各种真核细胞的信使核糖核蛋白颗粒中。通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳、等电聚焦以及用金黄色葡萄球菌蛋白酶进行有限蛋白水解后的肽图谱分析，确定了分子量为100000的信使核糖核蛋白蛋白质与细胞质可溶性部分中存在的“100K”蛋白质的同一性。
