大鼠肝脏核不均一核糖核蛋白颗粒中一种分子量为110000的蛋白质的特性

Properties of a 110000 molecular weight protein in rat liver hnRNP particles.

作者信息

Schweiger A, Kostka G

出版信息

Mol Biol Rep. 1981 May 22;7(1-3):47-51. doi: 10.1007/BF00778732.

Abstract

Particles carrying heterogeneous nuclear RNA (30--40 S-particles) were isolated from rat liver nuclei and the particle proteins separated by sodium dodecylsulfate gel electrophoresis. Some properties of a 110000 molecular weight component (P 110/103) were studied in detail: (i) P 110/103 was labeled to a 4-5 times higher specific activity than the major particle proteins in the presence of [14C]-amino acids in vivo. (ii) In nuclei incubated with [3H]- or [32P]-nicotinamide adenine dinucleotide P 110/103 was labeled presumably by ADP-ribosylation. (iii) A protein with the same molecular weight as P 110/103 and isolated from the nuclear extract by affinity chromatography was phosphorylated in vitro.

摘要

从大鼠肝细胞核中分离出携带不均一核RNA的颗粒（30 - 40S颗粒），并用十二烷基硫酸钠凝胶电泳分离颗粒蛋白。对一种分子量为110000的组分（P110/103）的一些特性进行了详细研究：（i）在体内存在[14C] - 氨基酸的情况下，P110/103的标记比主要颗粒蛋白的比活性高4 - 5倍。（ii）在用[3H] - 或[32P] - 烟酰胺腺嘌呤二核苷酸孵育的细胞核中，P110/103可能通过ADP - 核糖基化进行标记。（iii）从核提取物中通过亲和色谱法分离出的一种与P110/103分子量相同的蛋白质在体外被磷酸化。