el-Ganzoury A L
J Clin Pathol. 1967 Nov;20(6):879-82. doi: 10.1136/jcp.20.6.879.
Smears on slides taken from 44 patients suspected of having smallpox were examined for the presence of smallpox antigens and from 15 of them for chickenpox antigens also, by the fluorescent antibody method, using the conventional ;sandwich' and the complement techniques. In seven, very strong non-specific fluorescence made the result unreadable. When staining for smallpox antigen results agreeing with the diagnoses established by other methods were obtained in 36 of 37, and when staining for chickenpox in 12 of 13 specimens examined. One false positive diagnosis of smallpox and one false negative of chickenpox were made. Because of intense non-specific staining no diagnosis could be made from other smears stored for three years. It was not possible to reach a diagnosis by examination of crust suspensions spread on slides. In five fresh specimens examined during the outbreak there was hardly any non-specific fluorescence and results could more easily be read. Morphological features as seen by fluorescence microscopy are described. The value of the method in the diagnosis of smallpox is discussed.
对取自44名疑似患有天花患者的玻片涂片进行检查，以检测天花抗原的存在，同时还对其中15名患者的涂片检查水痘抗原，采用荧光抗体法，运用传统的“夹心”法和补体技术。有7例出现非常强烈的非特异性荧光，致使结果无法判读。检测天花抗原时，37例中有36例的染色结果与通过其他方法确定的诊断相符；检测水痘抗原时，在所检查的13份标本中有12例相符。出现了1例假阳性天花诊断和1例假阴性水痘诊断。由于存在强烈的非特异性染色，对保存了三年的其他涂片无法做出诊断。通过检查涂在玻片上的痂皮悬液无法做出诊断。在疫情暴发期间检查了5份新鲜标本，几乎没有非特异性荧光，结果更容易判读。描述了荧光显微镜下观察到的形态学特征。讨论了该方法在天花诊断中的价值。
