Brennan P C, Fritz T E, Flynn R J
J Bacteriol. 1969 Jan;97(1):337-49. doi: 10.1128/jb.97.1.337-349.1969.
Pneumonia caused by Mycoplasma pulmonis and Pasteurella pneumotropica was studied in conventional, specific pathogen-free (SPF), and germ-free mice. When P. pneumotropica was serially passed in conventional mice, M. pulmonis, as well as P. pneumotropica, was recovered from mice with gross lesions. When M. pulmonis was serially passed in conventional mice, both organisms were recovered. SPF mice given a nasal instillation of M. pulmonis alone, P. pneumotropica alone, or a combination of the two developed pneumonia when both organisms were present. These findings suggested that both organisms contribute to typical murine pneumonia. That M. pulmonis might be an L form of P. pneumotropica was suggested because some SPF mice inoculated with either organism yielded both on culture. This possibility was investigated with mole per cent guanine plus cytosine (GC) content and nucleic acid hybridization techniques. The GC content of P. pneumotropica is 42.2 mole per cent and that of M. pulmonis is 28.6 mole per cent. No specific hybrids between deoxyribonucleic acid (DNA) from M. pulmonis and DNA from P. pneumotropica were detected. This and the wide disparity in GC content showed that M. pulmonis is not an L form of P. pneumotropica. In germ-free mice, intranasal instillation with either organism alone produced pneumonia. The lesions produced when each organism was inoculated independently were characterized by areas of consolidation with perivascular and peribronchial lymphocytic infiltration. Qualitatively, the lesions produced when both organisms were inoculated simultaneously more closely resembled those seen in naturally occurring murine pneumonia. Statistical analysis indicated that the quantitative effect of the two organisms was additive. The indirect fluorescent antibody technique was used to locate organisms in lung tissue sections. M. pulmonis localized in the bronchial epithelium and P. pneumotropica localized in the alveolar lesions.
在普通小鼠、无特定病原体（SPF）小鼠和无菌小鼠中研究了由肺支原体和嗜肺巴斯德氏菌引起的肺炎。当嗜肺巴斯德氏菌在普通小鼠中连续传代时，从有明显病变的小鼠中分离出了肺支原体和嗜肺巴斯德氏菌。当肺支原体在普通小鼠中连续传代时，两种菌都能被分离出来。单独经鼻接种肺支原体、单独接种嗜肺巴斯德氏菌或同时接种这两种菌的SPF小鼠，当两种菌都存在时会发生肺炎。这些发现表明这两种菌都与典型的鼠肺炎有关。有人提出肺支原体可能是嗜肺巴斯德氏菌的L型，因为接种了任何一种菌的一些SPF小鼠在培养时都能分离出这两种菌。用鸟嘌呤加胞嘧啶（GC）含量百分比和核酸杂交技术对这种可能性进行了研究。嗜肺巴斯德氏菌的GC含量为42.2摩尔百分比，肺支原体的GC含量为28.6摩尔百分比。未检测到肺支原体的脱氧核糖核酸（DNA）与嗜肺巴斯德氏菌的DNA之间有特异性杂交。这一点以及GC含量的巨大差异表明肺支原体不是嗜肺巴斯德氏菌的L型。在无菌小鼠中，单独经鼻接种任何一种菌都会引起肺炎。单独接种每种菌时产生的病变特征为实变区域伴有血管周围和支气管周围淋巴细胞浸润。定性地说，同时接种两种菌时产生的病变与自然发生的鼠肺炎中所见的病变更相似。统计分析表明这两种菌的定量作用是相加的。用间接荧光抗体技术在肺组织切片中定位细菌。肺支原体定位于支气管上皮，嗜肺巴斯德氏菌定位于肺泡病变处。
